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Journal of Clinical Microbiology, 04 1995, 937-939, Vol 33, No. 4
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Parasitic culture of buffy coat for diagnosis of visceral leishmaniasis in human immunodeficiency virus-infected patients

R Lopez-Velez, F Laguna, J Alvar, JA Perez-Molina, R Molina, P Martinez and J Villarrubia
Tropical Medicine and Clinical Parasitology Unit, Hospital Ramon y Cajal, Madrid, Spain.

Two samples of buffy coat from the peripheral blood of 25 human immunodeficiency virus-positive patients with proven visceral leishmaniasis, as determined with a bone marrow aspirate (stain and culture), were cultured onto Schneider's and Novy-McNeal-Nicolle media. Hemoculture positivity was 67%. The average growing time was 10 days. This is an easy, noninvasive, and sensitive technique.

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