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Journal of Clinical Microbiology, May 1995, 1192-1195, Vol 33, No. 5
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Long PCR-ribotyping of nontypeable Haemophilus influenzae

HC Smith-Vaughan, KS Sriprakash, JD Mathews and DJ Kemp
Menzies School of Health Research, Darwin, Northern Territory, Australia.

PCR-ribotyping, a new typing method based on long PCR, has been developed for nontypeable Haemophilus influenzae (NTHi). Ribosomal operons of NTHi were amplified by long PCR and were found to be highly polymorphic for internal HaeIII sites. The technique was applied to 49 isolates previously subjected to conventional ribotyping, and the two methods showed a high level of concordance for serial isolates from individual subjects. PCR-ribotyping provides a powerful new typing tool for strain characterization in epidemiological investigations of NTHi.

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