This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Stanley, J. Articles by Burnens, A. Search for Related Content PubMed PubMed Citation Articles by Stanley, J. Articles by Burnens, A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 05 1995, 1206-1211, Vol 33, No. 5
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Molecular subtyping scheme for Salmonella panama

J Stanley, N Baquar and A Burnens
Virus Reference Division, Central Public Health Laboratory, London, United Kingdom.

We describe a genotyping scheme for Salmonella panama. Defined probes specific for the 16S rRNA gene and the DNA insertion element IS200 were generated by PCR from S. panama and were used to probe genomic Southern blots made with enzymes selected to cut within and outside the probed sequences. Plasmid profiles were determined. The typeability and discriminatory power of the individual methods were compared. Ribotyping with 16S rRNA gene probe alone was slightly more discriminatory than phage typing, but unlike the latter, ribotyping was able to type all strains. IS200 profiling was the single most discriminatory method for S. panama, having an index of discrimination (D) of 0.8 and 100% typeability. Plasmid profiling, which had moderate discriminatory power but only 50% typeability, was valuable as an adjunct technique. The use of all three methods together or simply the combination of IS200 profiling with the two most discriminatory enzymes and plasmid profiling yielded a molecular typing scheme whose discriminatory power (D = 0.97) approached the maximum theoretical value. This should prove both useful and robust for epidemiological investigations of S. panama.

This article has been cited by other articles:

• Chen, T.-L., Thien, P.-F., Liaw, S.-C., Fung, C.-P., Siu, L. K. (2005). First Report of Salmonella enterica Serotype Panama Meningitis Associated with Consumption of Contaminated Breast Milk by a Neonate. J. Clin. Microbiol. 43: 5400-5402 [Abstract] [Full Text]  
• Miko, A., Guerra, B., Schroeter, A., Dorn, C., Helmuth, R. (2002). Molecular Characterization of Multiresistant d-Tartrate-Positive Salmonella enterica Serovar Paratyphi B Isolates. J. Clin. Microbiol. 40: 3184-3191 [Abstract] [Full Text]