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Journal of Clinical Microbiology, Jun 1995, 1548-1550, Vol 33, No. 6
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Selective utilization of DNA probes for identification of Mycobacterium species on the basis of cord formation in primary BACTEC 12B cultures

DA Kaminski and DJ Hardy
Department of Microbiology and Immunology, School of Medicine and Dentistry, University of Rochester Medical Center, New York 14642-8710, USA.

Primary BACTEC 12B cultures with serpentine cords observed in Kinyoun- stained smears were tested with a probe for Mycobacterium tuberculosis complex, while cultures without cords were tested with a probe for M. avium complex. The sensitivity, specificity, and positive and negative predictive values of cording for the presumptive identification of M. tuberculosis were 95, 95, 90, and 98%, respectively. With experience, the selection of a probe for testing of primary BACTEC 12B cultures on the basis of cord formation and history of tuberculosis can provide a rapid and reliable approach to the laboratory diagnosis of tuberculosis.

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