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Journal of Clinical Microbiology, 06 1995, 1632-1634, Vol 33, No. 6
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Prospective comparison of direct immunofluorescence and conventional staining methods for detection of Giardia and Cryptosporidium spp. in human fecal specimens

AJ Alles, MA Waldron, LS Sierra and AR Mattia
Department of Pathology, Massachusetts General Hospital, Boston 02114, USA.

In a prospective comparative study, 2,696 consecutive fresh stool specimens over the course of 1 year were examined for Giardia lamblia and Cryptosporidium parvum by using a direct immunofluorescent- monoclonal antibody stain (for unspun specimens) and conventional staining methods (chlorazol black E for Giardia cysts and modified Kinyoun acid-fast for Cryptosporidium oocysts). The direct immunofluorescent-monoclonal antibody method resulted in a significantly increased detection rate for both giardia (118 versus 79 specimens, 49.4%; P = 0.006) and cryptosporidia (39 versus 23 specimens, 69.6%; P = 0.055).

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