This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ueyama, T. Articles by Mogi, G. Search for Related Content PubMed PubMed Citation Articles by Ueyama, T. Articles by Mogi, G.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 07 1995, 1835-1838, Vol 33, No. 7
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

High incidence of Haemophilus influenzae in nasopharyngeal secretions and middle ear effusions as detected by PCR

T Ueyama, Y Kurono, K Shirabe, M Takeshita and G Mogi
Department of Otolaryngology, Oita Medical University, Japan.

PCR was used to detect Haemophilus influenzae in samples of nasopharyngeal secretion and middle ear effusion (MEE). Nasopharyngeal secretions were collected from 102 patients with otitis media with effusion and from 111 healthy subjects. Eighty samples of MEE were collected from patients with otitis media with effusion. A pair of primers was designed to amplify a DNA segment of the gene encoding P6 outer membrane protein of H. influenzae. The amplified PCR product was detected with an internal probe that hybridized specifically to the P6 DNA of H. influenzae. Samples of MEE and nasopharyngeal secretion were also examined by a conventional culture method. The incidence of P6 gene DNA in nasopharyngeal secretions detected by PCR was about two times higher than that of H. influenzae detected by the conventional culture. Culture-positive samples were all positive in the PCR test. In MEEs, the rate of detection of the P6 gene DNA target was about five times higher than that of H. influenzae detected by the culture method. All patients who had P6 gene DNA in MEEs were found to have the DNA in nasopharyngeal secretions. These findings suggest that the presence of H. influenzae in MEEs and in nasopharyngeal secretions is more common than previously reported.

This article has been cited by other articles:

• Kelly, D. M., Greene, C. M., Meachery, G., O'Mahony, M., Gallagher, P. M., Taggart, C. C., O'Neill, S. J., McElvaney, N. G. (2005). Endotoxin Up-regulates Interleukin-18: Potential Role for Gram-Negative Colonization in Sarcoidosis. Am. J. Respir. Crit. Care Med. 172: 1299-1307 [Abstract] [Full Text]  
• Moyse, E., Lyon, M., Cordier, G., Mornex, J.-F., Collet, L., Froehlich, P. (2000). Viral RNA in Middle Ear Mucosa and Exudates in Patients With Chronic Otitis Media With Effusion. Arch Otolaryngol Head Neck Surg 126: 1105-1110 [Abstract] [Full Text]  
• Hendolin, P. H., Paulin, L., Ylikoski, J. (2000). Clinically Applicable Multiplex PCR for Four Middle Ear Pathogens. J. Clin. Microbiol. 38: 125-132 [Abstract] [Full Text]  
• Ueyama, T., Gu, X.-X., Tsai, C.-M., Karpas, A. B., Lim, D. J. (1999). Identification of Common Lipooligosaccharide Types in Isolates from Patients with Otitis Media by Monoclonal Antibodies against Nontypeable Haemophilus influenzae 9274. CVI 6: 96-100 [Abstract] [Full Text]  
• Matar, G. M., Sidani, N., Fayad, M., Hadi, U. (1998). Two-Step PCR-Based Assay for Identification of Bacterial Etiology of Otitis Media with Effusion in Infected Lebanese Children. J. Clin. Microbiol. 36: 1185-1188 [Abstract] [Full Text]  
• Bakaletz, L. O., White, G. J., Post, J. C., Ehrlich, G. D. (1998). Blinded Multiplex PCR Analyses of Middle Ear and Nasopharyngeal Fluids from Chinchilla Models of Single- and Mixed-Pathogen-Induced Otitis Media. CVI 5: 219-224 [Abstract] [Full Text]  
• Rayner, M. G., Zhang, Y., Gorry, M. C., Chen, Y., Post, J. C., Ehrlich, G. D. (1998). Evidence of Bacterial Metabolic Activity in Culture-Negative Otitis Media With Effusion. JAMA 279: 296-299 [Abstract] [Full Text]