This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gomez-Flores, R. Articles by Mehta, R. T. Search for Related Content PubMed PubMed Citation Articles by Gomez-Flores, R. Articles by Mehta, R. T.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 07 1995, 1842-1846, Vol 33, No. 7
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Determination of MICs for Mycobacterium avium-M. intracellulare complex in liquid medium by a colorimetric method

R Gomez-Flores, S Gupta, R Tamez-Guerra and RT Mehta
University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.

We investigated the potential of a rapid colorimetric microassay based on the reduction of dimethylthiazol-diphenyltetrazolium bromide (MTT) for determining the growth of Mycobacterium avium-M. intracellulare complex (MAC) and MICs of clofazimine, resorcinomycin A, and the quinolone PD 127391 against MAC. The reduction of MTT was directly proportional to the number of viable bacteria. A comparison of the MTT reduction test with the [3H]glycerol uptake assay showed the former to possess higher analytical sensitivity for detecting MAC growth in microtiter plates. The MIT reduction test avoids the use of radioisotopes and costly material and equipment; it is reliable, reproducible, and convenient for rapid routine susceptibility testing of MAC.

This article has been cited by other articles:

• Castro-Garza, J., Barrios-Garcia, H. B., Cruz-Vega, D. E., Said-Fernandez, S., Carranza-Rosales, P., Molina-Torres, C. A., Vera-Cabrera, L. (2007). Use of a colorimetric assay to measure differences in cytotoxicity of Mycobacterium tuberculosis strains. J Med Microbiol 56: 733-737 [Abstract] [Full Text]  
• Pietila, M. P., Vohra, P. K., Sanyal, B., Wengenack, N. L., Raghavakaimal, S., Thomas, C. F. Jr. (2006). Cloning and Characterization of CYP51 from Mycobacterium avium. Am. J. Respir. Cell Mol. Bio. 35: 236-242 [Abstract] [Full Text]  
• Palomino, J.-C., Martin, A., Camacho, M., Guerra, H., Swings, J., Portaels, F. (2002). Resazurin Microtiter Assay Plate: Simple and Inexpensive Method for Detection of Drug Resistance in Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 46: 2720-2722 [Abstract] [Full Text]  
• Mshana, R. N., Tadesse, G., Abate, G., Miörner, H. (1998). Use of 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyl Tetrazolium Bromide for Rapid Detection of Rifampin-Resistant Mycobacterium tuberculosis. J. Clin. Microbiol. 36: 1214-1219 [Abstract] [Full Text]  
• Franzblau, S. G., Witzig, R. S., McLaughlin, J. C., Torres, P., Madico, G., Hernandez, A., Degnan, M. T., Cook, M. B., Quenzer, V. K., Ferguson, R. M., Gilman, R. H. (1998). Rapid, Low-Technology MIC Determination with Clinical Mycobacterium tuberculosis Isolates by Using the Microplate Alamar Blue Assay. J. Clin. Microbiol. 36: 362-366 [Abstract] [Full Text]