This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wilson, M. L. Articles by Reller, L. B. Search for Related Content PubMed PubMed Citation Articles by Wilson, M. L. Articles by Reller, L. B.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 09 1995, 2265-2270, Vol 33, No. 9
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Controlled evaluation of BacT/alert standard anaerobic and FAN anaerobic blood culture bottles for the detection of bacteremia and fungemia

ML Wilson, MP Weinstein, S Mirrett, LG Reimer, RJ Feldman, CR Chuard and LB Reller
Department of Pathology and Laboratory Service, Denver Health & Hospitals, Colorado 80204, USA.

FAN medium was formulated to improve microbial recovery, particularly for fastidious microorganisms and for microorganisms causing sepsis in patients receiving antimicrobial therapy. In a controlled clinical evaluation performed at four university-affiliated hospitals, FAN anaerobic bottles were compared with standard anaerobic bottles for yield, speed of detection of microbial growth, and detection of septic episodes. A total of 10,431 blood culture sets were received; both anaerobic bottles of 7,694 blood culture sets were adequately filled with blood. Altogether, 925 isolates were recovered: 557 that were the cause of sepsis, 99 that were indeterminate as the cause of sepsis, and 269 contaminants. More Staphylococcus aureus (P < 0.001), coagulase- negative staphylococci (P < 0.001), Escherichia coli (P < 0.02), and all microorganisms combined (P < 0.005) were recovered from FAN bottles; more nonfermentative gram-negative bacilli (P < 0.05), Torulopsis glabrata (P < 0.001), and other yeasts (P < 0.01) were recovered from standard bottles. Growth of S. aureus (P < 0.001), coagulase-negative staphylococci (P < 0.001), Enterococcus faecalis (P < 0.025), streptococci other than Streptococcus pneumoniae (P < 0.01), and all microorganisms combined (P < 0.001) was detected earlier in standard bottles; growth of more isolates of E. coli (P < 0.05) and anaerobic bacteria (P < 0.01) was detected earlier in FAN bottles. The mean times to detection were 14.2 and 16.1 h for standard and FAN bottles, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

• Grohs, P., Mainardi, J.-L., Podglajen, I., Hanras, X., Eckert, C., Buu-Hoi, A., Varon, E., Gutmann, L. (2007). Relevance of Routine Use of the Anaerobic Blood Culture Bottle. J. Clin. Microbiol. 45: 2711-2715 [Abstract] [Full Text]  
• Flayhart, D., Borek, A. P., Wakefield, T., Dick, J., Carroll, K. C. (2007). Comparison of BACTEC PLUS Blood Culture Media to BacT/Alert FA Blood Culture Media for Detection of Bacterial Pathogens in Samples Containing Therapeutic Levels of Antibiotics. J. Clin. Microbiol. 45: 816-821 [Abstract] [Full Text]  
• Riley, J. A., Heiter, B. J., Bourbeau, P. P. (2005). Comparative Recovery of Microorganisms from BacT/ALERT Plastic and Glass FA and FN Blood Culture Bottles. J. Clin. Microbiol. 43: 3244-3246 [Abstract] [Full Text]  
• Mirrett, S., Petti, C. A., Woods, C. W., Magadia, R., Weinstein, M. P., Reller, L. B. (2004). Controlled Clinical Comparison of the BacT/ALERT FN and the Standard Anaerobic SN Blood Culture Medium. J. Clin. Microbiol. 42: 4581-4585 [Abstract] [Full Text]  
• Weinstein, M. P. (2003). Blood Culture Contamination: Persisting Problems and Partial Progress. J. Clin. Microbiol. 41: 2275-2278 [Full Text]  
• Riley, J. A., Heiter, B. J., Bourbeau, P. P. (2003). Comparison of Recovery of Blood Culture Isolates from Two BacT/ALERT FAN Aerobic Blood Culture Bottles with Recovery from One FAN Aerobic Bottle and One FAN Anaerobic Bottle. J. Clin. Microbiol. 41: 213-217 [Abstract] [Full Text]  
• Wilson, M. L., Mirrett, S., Meredith, F. T., Weinstein, M. P., Scotto, V., Reller, L. B. (2001). Controlled Clinical Comparison of BACTEC Plus Anaerobic/F to Standard Anaerobic/F as the Anaerobic Companion Bottle to Plus Aerobic/F Medium for Culturing Blood from Adults. J. Clin. Microbiol. 39: 983-989 [Abstract] [Full Text]  
• van der Vorm, E. R., Dondorp, A. M., van Ketel, R. J., Dankert, J. (2000). Apparent Culture-Negative Prosthetic Valve Endocarditis Caused by Peptostreptococcus magnus. J. Clin. Microbiol. 38: 4640-4642 [Abstract] [Full Text]  
• BROOKE, C. J., MARGAWANI, K. R., PEARSON, A. K., RILEY, T. V., ROBERTSON, I. D., HAMPSON, D. J. (2000). Evaluation of blood culture systems for detection of the intestinal spirochaete Brachyspira (Serpulina) pilosicoli in human blood. J Med Microbiol 49: 1031-1036 [Abstract] [Full Text]  
• Kempf, V. A. J., Trebesius, K., Autenrieth, I. B. (2000). Fluorescent In Situ Hybridization Allows Rapid Identification of Microorganisms in Blood Cultures. J. Clin. Microbiol. 38: 830-838 [Abstract] [Full Text]  
• Bourbeau, P., Riley, J., Heiter, B. J., Master, R., Young, C., Pierson, C. (1998). Use of the BacT/Alert Blood Culture System for Culture of Sterile Body Fluids Other than Blood. J. Clin. Microbiol. 36: 3273-3277 [Abstract] [Full Text]  
• Doern, G. V., Barton, A., Rao, S. (1998). Controlled Comparative Evaluation of BacT/Alert FAN and ESP 80A Aerobic Media as Means for Detecting Bacteremia and Fungemia. J. Clin. Microbiol. 36: 2686-2689 [Abstract] [Full Text]