This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Struelens, M. J. Articles by Serruys, E. Search for Related Content PubMed PubMed Citation Articles by Struelens, M. J. Articles by Serruys, E.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 09 1995, 2395-2399, Vol 33, No. 9
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Evaluation of Rapid ATB Staph for 5-hour antimicrobial susceptibility testing of Staphylococcus aureus. Groupement pour le Depistage, L'Etude et la Prevention des Infections Hospitalieres-Groep ter Opsporing, Studie en Preventie van Infecties in de Ziekenhuizen

MJ Struelens, C Nonhoff, P van der Auwera, R Mertens and E Serruys
Service de Microbiologie, Hopital Erasme, Brussels, Belgium.

The accuracy of Rapid ATB Staph (bioMerieux, La Balme-Les Grottes, France) for detection of oxacillin resistance and for detection susceptibility to 11 other antimicrobial agents in 553 and 519 Staphylococcus aureus isolates, respectively, was evaluated by comparing results with those produced by oxacillin agar screen and agar dilution methods, respectively. Further characterization of isolates with discrepant results for oxacillin testing was done by PCR detection of the nuc and mecA genes. By oxacillin agar screening, there were 307 oxacillin-resistant and 246 oxacillin-susceptible isolates. Rapid ATB results were obtained in 5 h for 515 (93.2%) of the isolates tested. Rapid ATB showed 97.0% sensitivity for detection of oxacillin resistance, confirmed by the presence of the mecA gene. After repeat testing of isolates flagged by the ATB software as possible errors, sensitivity increased to 99% for oxacillin-resistant isolates. Essential agreement with agar dilution testing for susceptibility to amoxicillin-clavulanic acid, gentamicin, erythromycin, clindamycin, and ciprofloxacin, as estimated by Youden's J statistic, was > 0.90. Subpopulations of isolates with significantly increased MICs of amikacin, rifampin, and minocycline, indicating borderline susceptibility, were detected by Rapid ATB and categorized as resistant. Rapid ATB Staph showed adequate accuracy for detection within 5 h of the oxacillin- and multiple-drug-resistant S. aureus isolates currently prevalent in Belgium.

This article has been cited by other articles:

• van Griethuysen, A., Pouw, M., van Leeuwen, N., Heck, M., Willemse, P., Buiting, A., Kluytmans, J. (1999). Rapid Slide Latex Agglutination Test for Detection of Methicillin Resistance in Staphylococcus aureus. J. Clin. Microbiol. 37: 2789-2792 [Abstract] [Full Text]  
• Frebourg, N., Nouet, D, Lemee, L, Martin, E, Lemeland, J. (1998). Comparison of ATB staph, rapid ATB staph, Vitek, and E-test methods for detection of oxacillin heteroresistance in staphylococci possessing mecA [In Process Citation]. J. Clin. Microbiol. 36: 52-57 [Abstract] [Full Text]