This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Calder, J. A.
Right arrow Articles by Dame, J. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Calder, J. A.
Right arrow Articles by Dame, J. B.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, Nov 1996, 2748-2755, Vol 34, No. 11
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Monitoring Babesia bovis infections in cattle by using PCR-based tests

JA Calder, GR Reddy, L Chieves, CH Courtney, R Littell, JR Livengood, RA Norval, C Smith and JB Dame
Department of Pathobiology, College of Veterinary Medicine, University of Florida, Gainesville 32611, USA. Cald103w@wonder.em.cdc.gov

The sensitivity and specificity of PCR tests based on the small-subunit rRNA gene sequence of Babesia bovis were compared in a blind study of experimentally infected cattle with the corresponding parameters of the complement fixation (CF) test currently used in the United States to screen for bovine babesiosis. Cattle were experimentally infected with a single inoculum of a cloned laboratory strain of B. bovis. Blood samples were collected and tested over a period covering from the day of infection to 10 months postinfection. The level of parasitemia (percent infected erythrocytes) present in each sample was estimated from test results and was plotted as a function of time postinfection. These data are the first describing the course of infection by methods capable of detecting parasitemias in the range of 10(-7)%, which frequently occur in the carrier state. Parasitemias in the samples tested strongly influenced the sensitivity and negative predictive value of the PCR-based tests which varied with time postinfection. The average sensitivities of the three PCR-based tests for B. bovis ranged from 58 to 70% for a single determination, while the sensitivity of the CF test was only 6%. Both PCR-based and CF tests for B. bovis had high specificity values ranging from 96 to 100%.


This article has been cited by other articles:

  • Kim, C., Iseki, H., Herbas, M. S., Yokoyama, N., Suzuki, H., Xuan, X., Fujisaki, K., Igarashi, I. (2007). Development of Taqman-Based Real-Time PCR Assays for Diagnostic Detection of Babesia bovis and Babesia bigemina. Am J Trop Med Hyg 77: 837-841 [Abstract] [Full Text]  
  • Howell, J. M., Ueti, M. W., Palmer, G. H., Scoles, G. A., Knowles, D. P. (2007). Persistently Infected Calves as Reservoirs for Acquisition and Transovarial Transmission of Babesia bovis by Rhipicephalus (Boophilus) microplus. J. Clin. Microbiol. 45: 3155-3159 [Abstract] [Full Text]  
  • Howell, J. M., Ueti, M. W., Palmer, G. H., Scoles, G. A., Knowles, D. P. (2007). Transovarial Transmission Efficiency of Babesia bovis Tick Stages Acquired by Rhipicephalus (Boophilus) microplus during Acute Infection. J. Clin. Microbiol. 45: 426-431 [Abstract] [Full Text]  
  • Birkenheuer, A. J., Levy, M. G., Stebbins, M., Poore, M., Breitschwerdt, E. (2003). Serosurvey of AntiBabesia Antibodies in Stray Dogs and American Pit Bull Terriers and American Staffordshire Terriers From North Carolina. Journal of the American Animal Hospital Association 39: 551-557 [Abstract] [Full Text]  
  • Birkenheuer, A. J., Levy, M. G., Breitschwerdt, E. B. (2003). Development and Evaluation of a Seminested PCR for Detection and Differentiation of Babesia gibsoni (Asian Genotype) and B. canis DNA in Canine Blood Samples. J. Clin. Microbiol. 41: 4172-4177 [Abstract] [Full Text]  
  • Tebele, N., Skilton, R. A., Katende, J., Wells, C. W., Nene, V., McElwain, T., Morzaria, S. P., Musoke, A. J. (2000). Cloning, Characterization, and Expression of a 200-Kilodalton Diagnostic Antigen of Babesia bigemina. J. Clin. Microbiol. 38: 2240-2247 [Abstract] [Full Text]  
  • O'Connor, R. M., Allred, D. R. (2000). Selection of Babesia bovis-Infected Erythrocytes for Adhesion to Endothelial Cells Coselects for Altered Variant Erythrocyte Surface Antigen Isoforms. J. Immunol. 164: 2037-2045 [Abstract] [Full Text]  
  • O'Connor, R. M., Long, J. A., Allred, D. R. (1999). Cytoadherence of Babesia bovis-Infected Erythrocytes to Bovine Brain Capillary Endothelial Cells Provides an In Vitro Model for Sequestration. Infect. Immun. 67: 3921-3928 [Abstract] [Full Text]  
  • Gubbels, J. M., de Vos, A. P., van der Weide, M., Viseras, J., Schouls, L. M., de Vries, E., Jongejan, F. (1999). Simultaneous Detection of Bovine Theileria and Babesia Species by Reverse Line Blot Hybridization. J. Clin. Microbiol. 37: 1782-1789 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»