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Journal of Clinical Microbiology, 12 1996, 3226-3229, Vol 34, No. 12
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Molecular epidemiology provides evidence of genotypic heterogeneity of multidrug-resistant Pseudomonas aeruginosa serotype O:12 outbreak isolates from a pediatric hospital

E Bingen, S Bonacorsi, P Rohrlich, M Duval, S Lhopital, N Brahimi, E Vilmer and RV Goering
Service de Microbiologie, Hopital Robert Debre, Paris, France.

Ribotyping randomly amplified polymorphic DNA analysis, and pulsed- field gel electrophoresis were used for the epidemiologic evaluation of eight Pseudomonas aeruginosa O:12 isolates obtained from eight children and two P. aeruginosa O:12 environmental isolates from a hematology ward. Randomly amplified polymorphic DNA analysis and pulsed-field gel electrophoresis were able to discriminate isolates that were indistinguishable by biochemical typing, O serotyping or ribotyping.


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