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Journal of Clinical Microbiology, 04 1996, 959-961, Vol 34, No. 4
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Molecular epidemiology of Escherichia coli O157:H7 by pulsed-field gel electrophoresis and comparison with that by bacteriophage typing

U Krause, FM Thomson-Carter and TH Pennington
Department of Medical Microbiology, Medical School, Foresterhill, Aberdeen, United Kingdom.

One hundred twenty-four Escherichia coli O157:H7 isolates were characterized by pulse-field gel electrophoresis, bacteriophage typing, and PCR of verotoxin genes. Diversity indices obtained--0.786 for phage types and 0.987 for pulsed-field gel electrophoresis types-- demonstrated that phage typing falls below the critical value (0.9) required for confident interpretation of results.


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