This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by de Lencastre, H.
Right arrow Articles by Tomasz, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by de Lencastre, H.
Right arrow Articles by Tomasz, A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, Sep 1996, 2121-2124, Vol 34, No. 9
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Methicillin-resistant Staphylococcus aureus isolates recovered from a New York City hospital: analysis by molecular fingerprinting techniques

H de Lencastre, A de Lencastre and A Tomasz
Rockefeller University, New York, New York 10021, USA.

Fifty-five methicillin-resistant Staphylococcus aureus (MRSA) isolates collected at New York Hospital Medical Center of Queens in 1989 were analyzed by molecular fingerprinting techniques. Close to 70% of these isolates (38 of 55) shared a common pulsed-field gel electrophoretic pattern, carried the same mecA gene polymorph type II, were free of the transposon Tn554, and would not react with a mecI-specific gene probe. An additional five isolates shared all properties of the major MRSA clone except that they carried mecA gene polymorph type III. All these isolates had an extremely heterogeneous methicillin resistance phenotype that belonged to population analysis profile class 1 or 2. The rest of the 12 MRSA isolates showed a variety of chromosomal pulsed- field gel electrophoretic patterns that carried different mecA polymorphs and that also gave positive reactions with DNA probes for Tn554 and for the mecI gene. The molecular features of the majority MRSA clone suggest that it is an archaic MRSA isolate similar in features to early MRSA isolates recovered in the 1960s.


This article has been cited by other articles:

  • Conceicao, T., Aires de Sousa, M., de Lencastre, H. (2009). Staphylococcal Interspersed Repeat Unit Typing of Staphylococcus aureus: Evaluation of a New Multilocus Variable-Number Tandem-Repeat Analysis Typing Method. J. Clin. Microbiol. 47: 1300-1308 [Abstract] [Full Text]  
  • Kwon, N. H., Park, K. T., Moon, J. S., Jung, W. K., Kim, S. H., Kim, J. M., Hong, S. K., Koo, H. C., Joo, Y. S., Park, Y. H. (2005). Staphylococcal cassette chromosome mec (SCCmec) characterization and molecular analysis for methicillin-resistant Staphylococcus aureus and novel SCCmec subtype IVg isolated from bovine milk in Korea. J Antimicrob Chemother 56: 624-632 [Abstract] [Full Text]  
  • Gomes, A. R., Vinga, S., Zavolan, M., de Lencastre, H. (2005). Analysis of the Genetic Variability of Virulence-Related Loci in Epidemic Clones of Methicillin-Resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 49: 366-379 [Abstract] [Full Text]  
  • Mundy, L. M., Sahm, D. F., Gilmore, M. (2000). Relationships between Enterococcal Virulence and Antimicrobial Resistance. Clin. Microbiol. Rev. 13: 513-522 [Abstract] [Full Text]  
  • Shimizu, A., Fujita, M., Igarashi, H., Takagi, M., Nagase, N., Sasaki, A., Kawano, J. (2000). Characterization of Staphylococcus aureus Coagulase Type VII Isolates from Staphylococcal Food Poisoning Outbreaks (1980-1995) in Tokyo, Japan, by Pulsed-Field Gel Electrophoresis. J. Clin. Microbiol. 38: 3746-3749 [Abstract] [Full Text]  
  • Melter, O., Santos Sanches, I., Schindler, J., Aires de Sousa, M., Mato, R., Kovárova, V., Zemlicková, H., de Lencastre, H. (1999). Methicillin-Resistant Staphylococcus aureus Clonal Types in the Czech Republic. J. Clin. Microbiol. 37: 2798-2803 [Abstract] [Full Text]  
  • Sá-Leão, R., Santos Sanches, I., Dias, D., Peres, I., Barros, R. M., de Lencastre, H. (1999). Detection of an Archaic Clone of Staphylococcus aureus with Low-Level Resistance to Methicillin in a Pediatric Hospital in Portugal and in International Samples: Relics of a Formerly Widely Disseminated Strain?. J. Clin. Microbiol. 37: 1913-1920 [Abstract] [Full Text]  
  • Seguin, J. C., Walker, R. D., Caron, J. P., Kloos, W. E., George, C. G., Hollis, R. J., Jones, R. N., Pfaller, M. A. (1999). Methicillin-Resistant Staphylococcus aureus Outbreak in a Veterinary Teaching Hospital: Potential Human-to-Animal Transmission. J. Clin. Microbiol. 37: 1459-1463 [Abstract] [Full Text]  
  • Givney, R., Vickery, A., Holliday, A., Pegler, M., Benn, R. (1998). Evolution of an Endemic Methicillin-Resistant Staphylococcus aureus Population in an Australian Hospital from 1967 to 1996. J. Clin. Microbiol. 36: 552-556 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»