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Journal of Clinical Microbiology, Sep 1996, 2290-2291, Vol 34, No. 9
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Modified India ink preparation for Cryptococcus neoformans in cerebrospinal fluid specimens

R Zerpa, L Huicho and A Guillen
Universidad Nacional Mayor de San Marcos, Lima, Peru.

A novel modified India ink technique for the diagnosis of Cryptococcus neoformans in cerebrospinal fluid specimens is described. It employs 2% chromium mercury and India ink. This technique allows a clear identification of some external and internal structures of the organism. Three layers from the outer capsule that have previously been discerned only by electron microscopy are distinguished.




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