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Journal of Clinical Microbiology, 01 1997, 213-216, Vol 35, No. 1
D Peele, J Bradfield, W Pryor and S Vore
This study compared the abilities of API 20E and BBL Crystal E/NF
identification systems to correctly identify human and animal source
gram-negative bacilli of known identifications, as provided by the American
Type Culture Collection, Rockville, Md., and the Research Diagnostic and
Investigative Laboratory, Columbia, Mo. Also addressed in the comparison
are the cost, the relative ease of performing and interpreting the tests,
and the potential problems surrounding each system. The two systems were
comparable in terms of their respective costs and abilities to identify the
bacteria tested. The cost per test was calculated as $4.69 for API 20E and
$4.62 for Crystal E/NF. Of the animal source bacteria tested, Crystal E/NF
identified 68% to the correct genus and species and 90% to the correct
genus or group. The remaining 10% of the animal source bacteria were
unidentified by Crystal. Human source bacteria tested by BBL Crystal E/NF
gave very similar results: 47% correctly identified to genus and species,
90% correctly identified to genus or group, 7% unidentified, and 3%
incorrectly identified. API 20E results were as follows for animal source
bacteria: 53% correctly identified to genus and species, 76% correctly
identified to genus or group, and 24% unidentified; the results for human
source bacteria were as follows: 40% correctly identified to genus and
species, 83% correctly identified to genus or group, and 17% unidentified.
API 20E has a slightly more labor- intensive protocol for setting up the
test than BBL Crystal E/NF but produced fewer questionable results.
Copyright © 1997 by the American Society for Microbiology. All rights reserved.
Comparison of identifications of human and animal source gram-negative bacteria by API 20E and crystal E/NF systems
Department of Comparative Medicine, East Carolina University School of Medicine, Greenville, North Carolina 27858, USA.
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