This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wenisch, C. Articles by Georgopoulos, A. Search for Related Content PubMed PubMed Citation Articles by Wenisch, C. Articles by Georgopoulos, A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, Jan 1997, 5-10, Vol 35, No. 1
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Rapid susceptibility testing of fungi by flow cytometry using vital staining

C Wenisch, KF Linnau, B Parschalk, K Zedtwitz-Liebenstein and A Georgopoulos
Department of Infectious Disease, Internal Medicine I, University Hospital of Vienna, Austria.

A 1-h assay for antifungal susceptibility testing measuring the impairment of fungal metabolic activity was developed. Yeast viability was analyzed by flow cytometry with a novel fluorescent probe, FUN-1, which emits a red fluorescence when the yeast is metabolically active. For nine Candida albicans strains tested, this method yielded results comparable to those obtained by the standard M27 procedure for amphotericin B, flucytosine, fluconazole, and ketoconazole. Whether the flow cytometry antifungal susceptibility test results correlate with the in vivo activities of the drugs remains to determined.

This article has been cited by other articles:

• Pina-Vaz, C., Costa-de-Oliveira, S., Rodrigues, A. G., Espinel-Ingroff, A. (2005). Comparison of Two Probes for Testing Susceptibilities of Pathogenic Yeasts to Voriconazole, Itraconazole, and Caspofungin by Flow Cytometry. J. Clin. Microbiol. 43: 4674-4679 [Abstract] [Full Text]  
• Mitchell, M., Hudspeth, M., Wright, A. (2005). Flow Cytometry Susceptibility Testing for the Antifungal Caspofungin. J. Clin. Microbiol. 43: 2586-2589 [Abstract] [Full Text]  
• Chaturvedi, V., Ramani, R., Pfaller, M. A. (2004). Collaborative Study of the NCCLS and Flow Cytometry Methods for Antifungal Susceptibility Testing of Candida albicans. J. Clin. Microbiol. 42: 2249-2251 [Abstract] [Full Text]  
• Liu, Y., Tortora, G., Ryan, M. E., Lee, H.-M., Golub, L. M. (2002). Potato Dextrose Agar Antifungal Susceptibility Testing for Yeasts and Molds: Evaluation of Phosphate Effect on Antifungal Activity of CMT-3. Antimicrob. Agents Chemother. 46: 1455-1461 [Abstract] [Full Text]  
• Marr, K. A., Koudadoust, M., Black, M., Balajee, S. A. (2001). Early Events in Macrophage Killing of Aspergillus fumigatus Conidia: New Flow Cytometric Viability Assay. CVI 8: 1240-1247 [Abstract] [Full Text]  
• Rex, J. H., Pfaller, M. A., Walsh, T. J., Chaturvedi, V., Espinel-Ingroff, A., Ghannoum, M. A., Gosey, L. L., Odds, F. C., Rinaldi, M. G., Sheehan, D. J., Warnock, D. W. (2001). Antifungal Susceptibility Testing: Practical Aspects and Current Challenges. Clin. Microbiol. Rev. 14: 643-658 [Abstract] [Full Text]  
• Wenisch, C., Moore, C. B., Krause, R., Presterl, E., Pichna, P., Denning, D. W. (2001). Antifungal Susceptibility Testing of Fluconazole by Flow Cytometry Correlates with Clinical Outcome. J. Clin. Microbiol. 39: 2458-2462 [Abstract] [Full Text]  
• Chang, H. C., Chang, J. J., Chan, S. H., Huang, A. H., Wu, T. L., Lin, M. C., Chang, T. C. (2001). Evaluation of Etest for Direct Antifungal Susceptibility Testing of Yeasts in Positive Blood Cultures. J. Clin. Microbiol. 39: 1328-1333 [Abstract] [Full Text]  
• PINA-VAZ, C., SANSONETTY, F., RODRIGUES, A. G., COSTA-DE-OLIVEIRA, S., MARTINEZ-DE-OLIVEIRA, J., FONSECA, A. F. (2001). Susceptibility to fluconazole of Candida clinical isolates determined by FUN-1 staining with flow cytometry and epifluorescence microscopy. J Med Microbiol 50: 375-382 [Abstract] [Full Text]  
• Lass-Flörl, C., Nagl, M., Speth, C., Ulmer, H., Dierich, M. P., Würzner, R. (2001). Studies of In Vitro Activities of Voriconazole and Itraconazole against Aspergillus Hyphae Using Viability Staining. Antimicrob. Agents Chemother. 45: 124-128 [Abstract] [Full Text]  
• Ramani, R., Chaturvedi, V. (2000). Flow Cytometry Antifungal Susceptibility Testing of Pathogenic Yeasts other than Candida albicans and Comparison with the NCCLS Broth Microdilution Test. Antimicrob. Agents Chemother. 44: 2752-2758 [Abstract] [Full Text]  
• Alvarez-Barrientos, A., Arroyo, J., Canton, R., Nombela, C., Sanchez-Perez, M. (2000). Applications of Flow Cytometry to Clinical Microbiology. Clin. Microbiol. Rev. 13: 167-195 [Abstract] [Full Text]  
• Chang Chang, H., Jung Chang, J., Huey Huang, A., Chain Chang, T. (2000). Evaluation of a Capacitance Method for Direct Antifungal Susceptibility Testing of Yeasts in Positive Blood Cultures. J. Clin. Microbiol. 38: 971-976 [Abstract] [Full Text]  
• Arthington-Skaggs, B. A., Jradi, H., Desai, T., Morrison, C. J. (1999). Quantitation of Ergosterol Content: Novel Method for Determination of Fluconazole Susceptibility of Candida albicans. J. Clin. Microbiol. 37: 3332-3337 [Abstract] [Full Text]  
• Gottfredsson, M., Erlendsdóttir, H., Sigfússon, A., Gudmundsson, S. (1998). Characteristics and Dynamics of Bacterial Populations during Postantibiotic Effect Determined by Flow Cytometry. Antimicrob. Agents Chemother. 42: 1005-1011 [Abstract] [Full Text]