This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ohara-Nemoto, Y. Articles by Kaneko, M. Search for Related Content PubMed PubMed Citation Articles by Ohara-Nemoto, Y. Articles by Kaneko, M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 10 1997, 2458-2463, Vol 35, No. 10
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Identification of Abiotrophia adiacens and Abiotrophia defectiva by 16S rRNA gene PCR and restriction fragment length polymorphism analysis

Y Ohara-Nemoto, S Tajika, M Sasaki and M Kaneko
Department of Microbiology, School of Dentistry, Iwate Medical University, Morioka, Japan. ynemoto@iwate-med.ac.jp

Abiotrophia adiacens and Abiotrophia defectiva, previously referred to as nutritionally variant streptococci, Streptococcus adjacens and Streptococcus defectivus, respectively, are causes of infective endocarditis. We describe a method of identifying these two species and also of distinguishing them from 15 other major etiological pathogens of infective endocarditis by means of 16S rRNA gene PCR amplification followed by restriction fragment length polymorphism analysis (PCR- RFLP). The 16S rRNA genes were successfully amplified with a set of universal primers from all 17 species of bacteria examined, including viridans group streptococci. The RFLP patterns of A. adiacens and A. defectiva obtained by HaeIII or MspI digestion were readily distinguished from each other and from those of other bacteria. When PCR analysis was performed with the supernatant of a suspension of a boiled colony, the 16S rRNA genes of 80 of 82 isolates (97%) of A. adiacens and all isolates (11 of 11) of A. defectiva were amplified. The HaeIII RFLP patterns of the isolates were the same as those of the corresponding type strains, although 28% of A. adiacens isolates revealed intraspecies polymorphism. The detection limit of this method was 0.1 pg of genomic DNA, as assessed by using the digoxigenin- labeling DNA detection system. Thus, the PCR-RFLP analysis that we developed is applicable for the routine detection of Abiotrophia from clinical specimens.

This article has been cited by other articles:

• Ohara-Nemoto, Y., Haraga, H., Kimura, S., Nemoto, T. K. (2008). Occurrence of staphylococci in the oral cavities of healthy adults and nasal oral trafficking of the bacteria. J Med Microbiol 57: 95-99 [Abstract] [Full Text]  
• Ohara-Nemoto, Y., Kishi, K., Satho, M., Tajika, S., Sasaki, M., Namioka, A., Kimura, S. (2005). Infective Endocarditis Caused by Granulicatella elegans Originating in the Oral Cavity. J. Clin. Microbiol. 43: 1405-1407 [Abstract] [Full Text]  
• Rovery, C., Greub, G., Lepidi, H., Casalta, J.-P., Habib, G., Collart, F., Raoult, D. (2005). PCR Detection of Bacteria on Cardiac Valves of Patients with Treated Bacterial Endocarditis. J. Clin. Microbiol. 43: 163-167 [Abstract] [Full Text]  
• Bosshard, P. P., Abels, S., Altwegg, M., Bottger, E. C., Zbinden, R. (2004). Comparison of Conventional and Molecular Methods for Identification of Aerobic Catalase-Negative Gram-Positive Cocci in the Clinical Laboratory. J. Clin. Microbiol. 42: 2065-2073 [Abstract] [Full Text]  
• Schlegel, L., Grimont, F., Grimont, P. A. D., Bouvet, A. (2003). Identification of Major Streptococcal Species by rrn-Amplified Ribosomal DNA Restriction Analysis. J. Clin. Microbiol. 41: 657-666 [Abstract] [Full Text]  
• Kanamoto, T., Sato, S., Inoue, M. (2000). Genetic Heterogeneities and Phenotypic Characteristics of Strains of the Genus Abiotrophia and Proposal of Abiotrophia para-adiacens sp. nov.. J. Clin. Microbiol. 38: 492-498 [Abstract] [Full Text]  
• Sato, S., Kanamoto, T., Inoue, M. (1999). Abiotrophia elegans Strains Comprise 8% of the Nutritionally Variant Streptococci Isolated from the Human Mouth. J. Clin. Microbiol. 37: 2553-2556 [Abstract] [Full Text]  
• Roggenkamp, A., Leitritz, L., Baus, K., Falsen, E., Heesemann, J. (1998). PCR for Detection and Identification of Abiotrophia spp.. J. Clin. Microbiol. 36: 2844-2846 [Abstract] [Full Text]