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Journal of Clinical Microbiology, 10 1997, 2514-2520, Vol 35, No. 10
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Clonal analysis and identification of epidemic strains of methicillin- resistant Staphylococcus aureus by antibiotyping and determination of protein A gene and coagulase gene polymorphisms

A Hoefnagels-Schuermans, WE Peetermans, MJ Struelens, S Van Lierde and J Van Eldere
Department of Microbiology and Immunology, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Belgium.

Forty-three methicillin-resistant Staphylococcus aureus (MRSA) isolates with known genetic and epidemiological relatedness and different degrees of transmission were analyzed by antibiotyping, protein A gene polymorphism analysis, and coagulase gene polymorphism analysis. The three typing systems were evaluated for their performance and convenience to define clones and to discriminate between epidemic MRSA (EMRSA) and sporadic MRSA (SMRSA). Antibiotyping and AluI restriction fragment length polymorphism analysis of the coagulase gene were able to define clones in the same way as DNA macrorestriction analysis (SmaI). However, both techniques presented disadvantages, making neither of them useful as a single typing method. Protein A gene polymorphism analysis appeared to be of no value for clonal analysis. None of the three typing methods was able to differentiate between EMRSA and SMRSA.

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