This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bascomb, S. Articles by Zimmer, B. L. Search for Related Content PubMed PubMed Citation Articles by Bascomb, S. Articles by Zimmer, B. L.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, Oct 1997, 2531-2536, Vol 35, No. 10
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Multicenter evaluation of the MicroScan Rapid Gram-Negative Identification Type 3 Panel

S Bascomb, SL Abbott, JD Bobolis, DA Bruckner, SJ Connell, SK Cullen, M Daugherty, D Glenn, JM Janda, SJ Lentsch, D Lindquist, PB Mayhew, DM Nothaft, JR Skinner, GB Williams, J Wong and BL Zimmer
Dade MicroScan Inc., West Sacramento, California 95691, USA.

The accuracy and performance of the revised MicroScan Rapid Gram- Negative Identification Type 3 Panel (Dade MicroScan Inc., West Sacramento, Calif.) were examined in a multicenter evaluation. The revised panel database includes data for 119 taxa covering a total of 150 species, with data for 12 new species added. Testing was performed in three phases: the efficacy, challenge, and reproducibility testing phases. A total of 405 fresh and stock gram-negative isolates comprising 54 species were tested in the efficacy phase; 96.8% of these species were identified correctly in comparison to the identification obtained either with the API 20E system (bioMerieux Vitek, Hazelwood, Mo.) or by the conventional tube method. The number of correctly identified isolates in the challenge phase, including new species added to the database, was 221 of 247, or 89.5%, in comparison to the number correctly identified by the conventional tube method. A total of 465 isolates were examined for intra- and interlaboratory identification reproducibility and gave an agreement of 464 of 465, or 99.8%. The overall reproducibility of each individual identification test or substrate was 14,373 of 14,384, or 99.9%. The new Rapid Gram-Negative Identification Type 3 Panel gave accurate and highly reproducible results in this multiple-laboratory evaluation.

This article has been cited by other articles:

• Janda, J. M., Abbott, S. L. (2006). The Genus Hafnia: from Soup to Nuts. Clin. Microbiol. Rev. 19: 12-28 [Abstract] [Full Text]  
• O'Hara, C. M., Brenner, F. W., Miller, J. M. (2000). Classification, Identification, and Clinical Significance of Proteus, Providencia, and Morganella. Clin. Microbiol. Rev. 13: 534-546 [Abstract] [Full Text]  
• O'Hara, C. M., Miller, J. M. (2000). Evaluation of the MicroScan Rapid Neg ID3 Panel for Identification of Enterobacteriaceae and Some Common Gram-Negative Nonfermenters. J. Clin. Microbiol. 38: 3577-3580 [Abstract] [Full Text]  
• Rodríguez, L. A., Vivas, J., Gallardo, C. S., Acosta, F., Barbeyto, L., Real, F. (1999). Identification of Hafnia alvei with the MicroScan WalkAway System. J. Clin. Microbiol. 37: 4186-4188 [Abstract] [Full Text]  
• Brenner, D. J., O'Hara, C. M., Grimont, P. A. D., Janda, J. M., Falsen, E., Aldova, E., Ageron, E., Schindler, J., Abbott, S. L., Steigerwalt, A. G. (1999). Biochemical Identification of Citrobacter Species Defined by DNA Hybridization and Description of Citrobacter gillenii sp. nov. (Formerly Citrobacter Genomospecies 10) and Citrobacter murliniae sp. nov. (Formerly Citrobacter Genomospecies 11). J. Clin. Microbiol. 37: 2619-2624 [Abstract] [Full Text]  
• Bascomb, S., Manafi, M. (1998). Use of Enzyme Tests in Characterization and Identification of Aerobic and Facultatively Anaerobic Gram-Positive Cocci. Clin. Microbiol. Rev. 11: 318-340 [Abstract] [Full Text]