This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nordentoft, S. Articles by Wegener, H. C. Search for Related Content PubMed PubMed Citation Articles by Nordentoft, S. Articles by Wegener, H. C.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 10 1997, 2642-2648, Vol 35, No. 10
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Evaluation of a fluorescence-labelled oligonucleotide probe targeting 23S rRNA for in situ detection of Salmonella serovars in paraffin- embedded tissue sections and their rapid identification in bacterial smears

S Nordentoft, H Christensen and HC Wegener
Danish Veterinary Laboratory, Copenhagen V. sn@svs.dk

A method for the detection of Salmonella based on fluorescence in situ hybridization (FISH) has been developed and applied for the direct detection of Salmonella in pure cultures and in formalin-fixed, paraffin-embedded tissue sections. On the basis of the 23S rRNA gene sequences representing all of the S. enterica subspecies and S. bongori, an 18-mer oligonucleotide probe was selected. The specificity of the probe was tested by in situ hybridization to bacterial cell smears of pure cultures. Forty-nine of 55 tested Salmonella serovars belonging to subspecies I, II, IIIb, IV, and VI hybridized with the probe. The probe did not hybridize to serovars from subspecies IIIa (S. arizonae) or to S. bongori. No cross-reaction to 64 other strains of the family Enterobacteriaceae or 18 other bacterial strains outside this family was observed. The probe was tested with sections of formalin-fixed, paraffin-embedded tissue from experimentally infected mice or from animals with a history of clinical salmonellosis. In these tissue sections the probe hybridized specifically to Salmonella serovars, allowing for the detection of single bacterial cells. The development of a fluorescence-labelled specific oligonucleotide probe makes the FISH technique a promising tool for the rapid identification of S. enterica in bacterial smears, as well as for the detection of S. enterica in histological tissue sections.

This article has been cited by other articles:

• Jensen, T. K., Boye, M., Moller, K. (2004). Extensive intestinal spirochaetosis in pigs challenged with Brachyspira pilosicoli. J Med Microbiol 53: 309-312 [Abstract] [Full Text]  
• Mbuthia, P. G., Christensen, H., Boye, M., Petersen, K. M. D., Bisgaard, M., Nyaga, P. N., Olsen, J. E. (2001). Specific Detection of Pasteurella multocida in Chickens with Fowl Cholera and in Pig Lung Tissues Using Fluorescent rRNA In Situ Hybridization. J. Clin. Microbiol. 39: 2627-2633 [Abstract] [Full Text]  
• Hald, B., Knudsen, K., Lind, P., Madsen, M. (2001). Study of the Infectivity of Saline-Stored Campylobacter jejuni for Day-Old Chicks. Appl. Environ. Microbiol. 67: 2388-2392 [Abstract] [Full Text]