This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cassol, S.
Right arrow Articles by Forbes, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cassol, S.
Right arrow Articles by Forbes, J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 11 1997, 2795-2801, Vol 35, No. 11
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Quantification of human immunodeficiency virus type 1 RNA from dried plasma spots collected on filter paper [In Process Citation]

S Cassol, MJ Gill, R Pilon, M Cormier, RF Voigt, B Willoughby and J Forbes
Ottawa General Hospital Research Institute and the University of Ottawa, Ontario, Canada.

To assess dried plasma spots (DPSs) as a source of material for virus quantification, human immunodeficiency virus type 1 (HIV-1) RNA levels were quantified in matched DPS and liquid plasma samples from 73 infected patients, including 5 neonates and 4 adult patients with acute HIV-1 infection. Quantifications were performed by commercially available assays (NASBA [nucleic acid sequence-based amplification] or Amplicor, or both). There was a strong correlation between HIV-1 RNA levels in plasma and DPSs. More importantly, there was no decline in HIV-1 RNA levels in DPSs stored for as long as 2 weeks at 20 degrees C. Similarly, storage of DPSs for 3 days at 37 degrees C resulted in no decrease in viral RNA levels. For patients with primary infection, the DPS method allowed for the measurement of RNA levels in plasma during the initial spike in the level of viremia and in the subsequent period of suppressed viral replication. DPS quantification was equally informative in the neonatal setting, with all five newborns showing HIV- 1 RNA loads of greater than 4.991 log10 copies/ml. We conclude that the viral RNA levels in DPSs are equivalent to those measured in fresh- frozen plasma. The ease and economy of DPS sampling, the minute volumes required, and the unexpected stability of dried RNA suggest that the use of DPSs will be particularly valuable for small-volume neonatal samples and large, population-based studies in which cold storage and transportation present special problems, as is often the case in developing countries. The ability to measure viral changes during primary infection suggests that the method will be useful for assessing vaccine efficacy in large field trials.


This article has been cited by other articles:

  • Lloyd, R. M. Jr., Burns, D. A., Huong, J. T., Mathis, R. L., Winters, M. A., Tanner, M., De La Rosa, A., Yen-Lieberman, B., Armstrong, W., Taege, A., McClernon, D. R., Wetshtein, J. L., Friedrich, B. M., Ferguson, M. R., O'Brien, W., Feorino, P. M., Holodniy, M. (2009). Dried-Plasma Transport Using a Novel Matrix and Collection System for Human Immunodeficiency Virus and Hepatitis C Virus Virologic Testing. J. Clin. Microbiol. 47: 1491-1496 [Abstract] [Full Text]  
  • Ikomey, G.M., Atashili, J., Okomo-Assoumou, M.C., Mesembe, M., Ndumbe, P.M. (2009). Dried Blood Spots Versus Plasma for the Quantification of HIV-1 RNA Using the Manual (PCR-ELISA) Amplicor Monitor HIV-1 Version 1.5 Assay in Yaounde, Cameroon. J Int Assoc Physicians AIDS Care (Chic Ill) 8: 181-184 [Abstract]  
  • Garrido, C., Zahonero, N., Corral, A., Arredondo, M., Soriano, V., de Mendoza, C. (2009). Correlation between Human Immunodeficiency Virus Type 1 (HIV-1) RNA Measurements Obtained with Dried Blood Spots and Those Obtained with Plasma by Use of Nuclisens EasyQ HIV-1 and Abbott RealTime HIV Load Tests. J. Clin. Microbiol. 47: 1031-1036 [Abstract] [Full Text]  
  • Monleau, M., Montavon, C., Laurent, C., Segondy, M., Montes, B., Delaporte, E., Boillot, F., Peeters, M. (2009). Evaluation of Different RNA Extraction Methods and Storage Conditions of Dried Plasma or Blood Spots for Human Immunodeficiency Virus Type 1 RNA Quantification and PCR Amplification for Drug Resistance Testing. J. Clin. Microbiol. 47: 1107-1118 [Abstract] [Full Text]  
  • Bland, R., Coovadia, H., Coutsoudis, A, Rollins, N., Newell, M. (2009). Cohort Profile: Mamanengane or the Africa Centre Vertical Transmission Study. Int J Epidemiol 0: dyp165v1-dyp165 [Full Text]  
  • Lakshmi, V., Sudha, T., Dandona, R., Teja, V. D., Kumar, G. A., Dandona, L. (2009). Application of human immunodeficiency virus type 1 BED enzyme immunoassay on dried blood spots in India. J Med Microbiol 58: 312-317 [Abstract] [Full Text]  
  • Land, A. M., Ball, T. B., Luo, M., Pilon, R., Sandstrom, P., Embree, J. E., Wachihi, C., Kimani, J., Plummer, F. A. (2008). Human Immunodeficiency Virus (HIV) Type 1 Proviral Hypermutation Correlates with CD4 Count in HIV-Infected Women from Kenya. J. Virol. 82: 8172-8182 [Abstract] [Full Text]  
  • Youngpairoj, A. S., Masciotra, S., Garrido, C., Zahonero, N., de Mendoza, C., Garcia-Lerma, J. G. (2008). HIV-1 drug resistance genotyping from dried blood spots stored for 1 year at 4{degrees}C. J Antimicrob Chemother 61: 1217-1220 [Abstract] [Full Text]  
  • Steegen, K., Luchters, S., Demecheleer, E., Dauwe, K., Mandaliya, K., Jaoko, W., Plum, J., Temmerman, M., Verhofstede, C. (2007). Feasibility of Detecting Human Immunodeficiency Virus Type 1 Drug Resistance in DNA Extracted from Whole Blood or Dried Blood Spots. J. Clin. Microbiol. 45: 3342-3351 [Abstract] [Full Text]  
  • Ayele, W., Schuurman, R., Messele, T., Dorigo-Zetsma, W., Mengistu, Y., Goudsmit, J., Paxton, W. A., de Baar, M. P., Pollakis, G. (2007). Use of Dried Spots of Whole Blood, Plasma, and Mother's Milk Collected on Filter Paper for Measurement of Human Immunodeficiency Virus Type 1 Burden. J. Clin. Microbiol. 45: 891-896 [Abstract] [Full Text]  
  • Barin, F., Meyer, L., Lancar, R., Deveau, C., Gharib, M., Laporte, A., Desenclos, J.-C., Costagliola, D. (2005). Development and Validation of an Immunoassay for Identification of Recent Human Immunodeficiency Virus Type 1 Infections and Its Use on Dried Serum Spots. J. Clin. Microbiol. 43: 4441-4447 [Abstract] [Full Text]  
  • Li, C.-C., Seidel, K. D., Coombs, R. W., Frenkel, L. M. (2005). Detection and Quantification of Human Immunodeficiency Virus Type 1 p24 Antigen in Dried Whole Blood and Plasma on Filter Paper Stored under Various Conditions. J. Clin. Microbiol. 43: 3901-3905 [Abstract] [Full Text]  
  • Herzmann, C., Karcher, H., Goulder, P. J.R., Blanckenberg, N., Dong, K., Lallemant, M., Jourdain, G., Le Coeur, S. (2004). Nevirapine plus Zidovudine to Prevent Mother-to-Child Transmission of HIV. NEJM 351: 2013-2015 [Full Text]  
  • Li, C.-C., Beck, I. A., Seidel, K. D., Frenkel, L. M. (2004). Persistence of Human Immunodeficiency Virus Type 1 Subtype B DNA in Dried-Blood Samples on FTA Filter Paper. J. Clin. Microbiol. 42: 3847-3849 [Abstract] [Full Text]  
  • Rahman, M., Goegebuer, T., De Leener, K., Maes, P., Matthijnssens, J., Podder, G., Azim, T., Van Ranst, M. (2004). Chromatography Paper Strip Method for Collection, Transportation, and Storage of Rotavirus RNA in Stool Samples. J. Clin. Microbiol. 42: 1605-1608 [Abstract] [Full Text]  
  • Brambilla, D., Jennings, C., Aldrovandi, G., Bremer, J., Comeau, A. M., Cassol, S. A., Dickover, R., Jackson, J. B., Pitt, J., Sullivan, J. L., Butcher, A., Grosso, L., Reichelderfer, P., Fiscus, S. A. (2003). Multicenter Evaluation of Use of Dried Blood and Plasma Spot Specimens in Quantitative Assays for Human Immunodeficiency Virus RNA: Measurement, Precision, and RNA Stability. J. Clin. Microbiol. 41: 1888-1893 [Abstract] [Full Text]  
  • Gordon, M., De Oliveira, T., Bishop, K., Coovadia, H. M., Madurai, L., Engelbrecht, S., Janse van Rensburg, E., Mosam, A., Smith, A., Cassol, S. (2003). Molecular Characteristics of Human Immunodeficiency Virus Type 1 Subtype C Viruses from KwaZulu-Natal, South Africa: Implications for Vaccine and Antiretroviral Control Strategies. J. Virol. 77: 2587-2599 [Abstract] [Full Text]  
  • Solmone, M., Girardi, E., Costa, F., Pucillo, L., Ippolito, G., Capobianchi, M. R. (2002). Simple and Reliable Method for Detection and Genotyping of Hepatitis C Virus RNA in Dried Blood Spots Stored at Room Temperature. J. Clin. Microbiol. 40: 3512-3514 [Abstract] [Full Text]  
  • Cao, J., Cousins, R. J. (2000). Metallothionein mRNA in Monocytes and Peripheral Blood Mononuclear Cells and in Cells from Dried Blood Spots Increases after Zinc Supplementation of Men. J. Nutr. 130: 2180-2187 [Abstract] [Full Text]  
  • Oelrichs, R. B., Shrestha, I. L., Anderson, D. A., Deacon, N. J. (2000). The Explosive Human Immunodeficiency Virus Type 1 Epidemic among Injecting Drug Users of Kathmandu, Nepal, Is Caused by a Subtype C Virus of Restricted Genetic Diversity. J. Virol. 74: 1149-1157 [Abstract] [Full Text]  
  • Lew, J., Reichelderfer, P., Fowler, M., Bremer, J., Carrol, R., Cassol, S., Chernoff, D., Coombs, R., Cronin, M., Dickover, R., Fiscus, S., Herman, S., Jackson, B., Kornegay, J., Kovacs, A., McIntosh, K., Meyer, W., Michael, N., Mofenson, L., Moye, J., Quinn, T., Robb, M., Vahey, M., Weiser, B., Yeghiazarian, T. (1998). Determinations of Levels of Human Immunodeficiency Virus Type 1 RNA in Plasma: Reassessment of Parameters Affecting Assay Outcome. J. Clin. Microbiol. 36: 1471-1479 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»