This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Meinersmann, R. J.
Right arrow Articles by Hiett, K. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Meinersmann, R. J.
Right arrow Articles by Hiett, K. L.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, Nov 1997, 2810-2814, Vol 35, No. 11
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Discrimination of Campylobacter jejuni isolates by fla gene sequencing [In Process Citation]

RJ Meinersmann, LO Helsel, PI Fields and KL Hiett
Agricultural Research Service, U.S. Department of Agriculture, Athens, Georgia 30604, USA. Rmeiners@asrr.arsusda.gov

Comparison of the entire coding sequence of flaA (1,764 nucleotides) from 15 isolates of Campylobacter jejuni showed two regions of high variability, one region approximately from base positions 700 to 1,450 and a short variable region (SVR) from base positions 450 to 600. Parsimony analysis of the SVR sequences yielded a dendrogram similar to that which was derived by analysis of the entire gene. PCR was used to generate templates, and the SVR was sequenced with primers constructed to hybridize to conserved flanking sequences. The SVRs of 22 isolates of C. jejuni from four outbreaks that have been well characterized and a larger panel of isolates from three additional outbreaks were sequenced. Analysis of the nucleotide sequences produced results that grouped the isolates very similarly to other subtyping techniques. Sequence data were also generated for isolates from three additional outbreaks. Categorizing the isolates by fla SVR DNA sequence placed them in epidemiologically relevant groups. Sequence analysis of the C. jejuni flaA SVR may be a useful tool for epidemiologic investigations and could complement or replace serotyping and other subtyping methods.


This article has been cited by other articles:

  • Cox, N. A., Richardson, L. J., Buhr, R. J., Fedorka-Cray, P. J. (2009). Campylobacter species occurrence within internal organs and tissues of commercial caged Leghorn laying hens. Poult. Sci. 88: 2449-2456 [Abstract] [Full Text]  
  • Elviss, N. C., Williams, L. K., Jorgensen, F., Chisholm, S. A., Lawson, A. J., Swift, C., Owen, R. J., Griggs, D. J., Johnson, M. M., Humphrey, T. J., Piddock, L. J. V. (2009). Amoxicillin therapy of poultry flocks: effect upon the selection of amoxicillin-resistant commensal Campylobacter spp.. J Antimicrob Chemother 64: 702-711 [Abstract] [Full Text]  
  • Korczak, B. M., Zurfluh, M., Emler, S., Kuhn-Oertli, J., Kuhnert, P. (2009). Multiplex Strategy for Multilocus Sequence Typing, fla Typing, and Genetic Determination of Antimicrobial Resistance of Campylobacter jejuni and Campylobacter coli Isolates Collected in Switzerland. J. Clin. Microbiol. 47: 1996-2007 [Abstract] [Full Text]  
  • Ragimbeau, C., Schneider, F., Losch, S., Even, J., Mossong, J. (2008). Multilocus Sequence Typing, Pulsed-Field Gel Electrophoresis, and fla Short Variable Region Typing of Clonal Complexes of Campylobacter jejuni Strains of Human, Bovine, and Poultry Origins in Luxembourg. Appl. Environ. Microbiol. 74: 7715-7722 [Abstract] [Full Text]  
  • Callicott, K. A., Harthardottir, H., Georgsson, F., Reiersen, J., Frithriksdottir, V., Gunnarsson, E., Michel, P., Bisaillon, J.-R., Kristinsson, K. G., Briem, H., Hiett, K. L., Needleman, D. S., Stern, N. J. (2008). Broiler Campylobacter Contamination and Human Campylobacteriosis in Iceland. Appl. Environ. Microbiol. 74: 6483-6494 [Abstract] [Full Text]  
  • Levesque, S., Frost, E., Arbeit, R. D., Michaud, S. (2008). Multilocus Sequence Typing of Campylobacter jejuni Isolates from Humans, Chickens, Raw Milk, and Environmental Water in Quebec, Canada. J. Clin. Microbiol. 46: 3404-3411 [Abstract] [Full Text]  
  • Piddock, L. J. V., Griggs, D., Johnson, M. M., Ricci, V., Elviss, N. C., Williams, L. K., Jorgensen, F., Chisholm, S. A., Lawson, A. J., Swift, C., Humphrey, T. J., Owen, R. J. (2008). Persistence of Campylobacter species, strain types, antibiotic resistance and mechanisms of tetracycline resistance in poultry flocks treated with chlortetracycline. J Antimicrob Chemother 62: 303-315 [Abstract] [Full Text]  
  • Colles, F. M., Dingle, K. E., Cody, A. J., Maiden, M. C. J. (2008). Comparison of Campylobacter Populations in Wild Geese with Those in Starlings and Free-Range Poultry on the Same Farm. Appl. Environ. Microbiol. 74: 3583-3590 [Abstract] [Full Text]  
  • Ridley, A. M., Allen, V. M., Sharma, M., Harris, J. A., Newell, D. G. (2008). Real-Time PCR Approach for Detection of Environmental Sources of Campylobacter Strains Colonizing Broiler Flocks. Appl. Environ. Microbiol. 74: 2492-2504 [Abstract] [Full Text]  
  • Elvers, K. T., Helps, C. R., Wassenaar, T. M., Allen, V. M., Newell, D. G. (2008). Development of a Strain-Specific Molecular Method for Quantitating Individual Campylobacter Strains in Mixed Populations. Appl. Environ. Microbiol. 74: 2321-2331 [Abstract] [Full Text]  
  • Balajee, S. A., Tay, S. T., Lasker, B. A., Hurst, S. F., Rooney, A. P. (2007). Characterization of a Novel Gene for Strain Typing Reveals Substructuring of Aspergillus fumigatus across North America. Eukaryot Cell 6: 1392-1399 [Abstract] [Full Text]  
  • Stern, N. J., Georgsson, F., Lowman, R., Bisaillon, J.-R., Reiersen, J., Callicott, K. A., Geirsdottir, M., Hrolfsdottir, R., Hiett, K. L., the Campy-on-Ice Consortium, (2007). Frequency and Enumeration of Campylobacter Species from Processed Broiler Carcasses by Weep and Rinse Samples. Poult. Sci. 86: 394-399 [Abstract] [Full Text]  
  • Djordjevic, S. P., Unicomb, L. E., Adamson, P. J., Mickan, L., Rios, R., the Australian Campylobacter Subtyping Study Group, (2007). Clonal Complexes of Campylobacter jejuni Identified by Multilocus Sequence Typing Are Reliably Predicted by Restriction Fragment Length Polymorphism Analyses of the flaA Gene. J. Clin. Microbiol. 45: 102-108 [Abstract] [Full Text]  
  • Price, E. P., Huygens, F., Giffard, P. M. (2006). Fingerprinting of Campylobacter jejuni by Using Resolution-Optimized Binary Gene Targets Derived from Comparative Genome Hybridization Studies. Appl. Environ. Microbiol. 72: 7793-7803 [Abstract] [Full Text]  
  • Callicott, K. A., Frithriksdottir, V., Reiersen, J., Lowman, R., Bisaillon, J.-R., Gunnarsson, E., Berndtson, E., Hiett, K. L., Needleman, D. S., Stern, N. J. (2006). Lack of Evidence for Vertical Transmission of Campylobacter spp. in Chickens. Appl. Environ. Microbiol. 72: 5794-5798 [Abstract] [Full Text]  
  • Price, E. P., Thiruvenkataswamy, V., Mickan, L., Unicomb, L., Rios, R. E., Huygens, F., Giffard, P. M. (2006). Genotyping of Campylobacter jejuni using seven single-nucleotide polymorphisms in combination with flaA short variable region sequencing.. J Med Microbiol 55: 1061-1070 [Abstract] [Full Text]  
  • Meinersmann, R. J., Phillips, R. W., Hiett, K. L., Fedorka-Cray, P. (2005). Differentiation of Campylobacter Populations as Demonstrated by Flagellin Short Variable Region Sequences. Appl. Environ. Microbiol. 71: 6368-6374 [Abstract] [Full Text]  
  • Fitch, B. R., Sachen, K. L., Wilder, S. R., Burg, M. A., Lacher, D. W., Khalife, W. T., Whittam, T. S., Young, V. B. (2005). Genetic Diversity of Campylobacter sp. Isolates from Retail Chicken Products and Humans with Gastroenteritis in Central Michigan. J. Clin. Microbiol. 43: 4221-4224 [Abstract] [Full Text]  
  • Clark, C. G., Bryden, L., Cuff, W. R., Johnson, P. L., Jamieson, F., Ciebin, B., Wang, G. (2005). Use of the Oxford Multilocus Sequence Typing Protocol and Sequencing of the Flagellin Short Variable Region To Characterize Isolates from a Large Outbreak of Waterborne Campylobacter sp. Strains in Walkerton, Ontario, Canada. J. Clin. Microbiol. 43: 2080-2091 [Abstract] [Full Text]  
  • Dingle, K. E., Colles, F. M., Falush, D., Maiden, M. C. J. (2005). Sequence Typing and Comparison of Population Biology of Campylobacter coli and Campylobacter jejuni. J. Clin. Microbiol. 43: 340-347 [Abstract] [Full Text]  
  • Klena, J. D., Parker, C. T., Knibb, K., Ibbitt, J. C., Devane, P. M. L., Horn, S. T., Miller, W. G., Konkel, M. E. (2004). Differentiation of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis by a Multiplex PCR Developed from the Nucleotide Sequence of the Lipid A Gene lpxA. J. Clin. Microbiol. 42: 5549-5557 [Abstract] [Full Text]  
  • Mellmann, A., Mosters, J., Bartelt, E., Roggentin, P., Ammon, A., Friedrich, A. W., Karch, H., Harmsen, D. (2004). Sequence-Based Typing of flaB Is a More Stable Screening Tool Than Typing of flaA for Monitoring of Campylobacter Populations. J. Clin. Microbiol. 42: 4840-4842 [Abstract] [Full Text]  
  • Duim, B., Godschalk, P. C. R., van den Braak, N., Dingle, K. E., Dijkstra, J. R., Leyde, E., van der Plas, J., Colles, F. M., Endtz, H. P., Wagenaar, J. A., Maiden, M. C. J., van Belkum, A. (2003). Molecular Evidence for Dissemination of Unique Campylobacter jejuni Clones in Curacao, Netherlands Antilles. J. Clin. Microbiol. 41: 5593-5597 [Abstract] [Full Text]  
  • Sails, A. D., Swaminathan, B., Fields, P. I. (2003). Utility of Multilocus Sequence Typing as an Epidemiological Tool for Investigation of Outbreaks of Gastroenteritis Caused by Campylobacter jejuni. J. Clin. Microbiol. 41: 4733-4739 [Abstract] [Full Text]  
  • Sails, A. D., Swaminathan, B., Fields, P. I. (2003). Clonal Complexes of Campylobacter jejuni Identified by Multilocus Sequence Typing Correlate with Strain Associations Identified by Multilocus Enzyme Electrophoresis. J. Clin. Microbiol. 41: 4058-4067 [Abstract] [Full Text]  
  • Hiett, K. L, Stern, N. J., Fedorka-Cray, P., Cox, N. A., Musgrove, M. T., Ladely, S. (2002). Molecular Subtype Analyses of Campylobacter spp. from Arkansas and California Poultry Operations. Appl. Environ. Microbiol. 68: 6220-6236 [Abstract] [Full Text]  
  • Karjalainen, T., Saumier, N., Barc, M.-C., Delmee, M., Collignon, A. (2002). Clostridium difficile Genotyping Based on slpA Variable Region in S-Layer Gene Sequence: an Alternative to Serotyping. J. Clin. Microbiol. 40: 2452-2458 [Abstract] [Full Text]  
  • Dingle, K. E., Van Den Braak, N., Colles, F. M., Price, L. J., Woodward, D. L., Rodgers, F. G., Endtz, H. P., Van Belkum, A., Maiden, M. C. J. (2001). Sequence Typing Confirms that Campylobacter jejuni Strains Associated with Guillain-Barre and Miller-Fisher Syndromes Are of Diverse Genetic Lineage, Serotype, and Flagella Type. J. Clin. Microbiol. 39: 3346-3349 [Abstract] [Full Text]  
  • Fitzgerald, C., Helsel, L. O., Nicholson, M. A., Olsen, S. J., Swerdlow, D. L., Flahart, R., Sexton, J., Fields, P. I. (2001). Evaluation of Methods for Subtyping Campylobacter jejuni during an Outbreak Involving a Food Handler. J. Clin. Microbiol. 39: 2386-2390 [Abstract] [Full Text]  
  • Suerbaum, S., Lohrengel, M., Sonnevend, A., Ruberg, F., Kist, M. (2001). Allelic Diversity and Recombination in Campylobacter jejuni. J. Bacteriol. 183: 2553-2559 [Abstract] [Full Text]  
  • Chen, H.-C., Stern, N. J. (2001). Competitive Exclusion of Heterologous Campylobacter spp. in Chicks. Appl. Environ. Microbiol. 67: 848-851 [Abstract] [Full Text]  
  • Meinersmann, R. J., Hiett, K. L. (2000). Concerted evolution of duplicate fla genes in Campylobacter. Microbiology 146: 2283-2290 [Abstract] [Full Text]  
  • de Boer, P., Duim, B., Rigter, A., van der Plas, J., Jacobs-Reitsma, W. F., Wagenaar, J. A. (2000). Computer-Assisted Analysis and Epidemiological Value of Genotyping Methods for Campylobacter jejuni and Campylobacter coli. J. Clin. Microbiol. 38: 1940-1946 [Abstract] [Full Text]  
  • Wassenaar, T. M., Newell, D. G. (2000). Genotyping of Campylobacter spp.. Appl. Environ. Microbiol. 66: 1-9 [Full Text]  
  • Broman, T., Bergström, S., On, S. L. W., Palmgren, H., McCafferty, D. J., Sellin, M., Olsen, B. (2000). Isolation and Characterization of Campylobacter jejuni subsp. jejuni from Macaroni Penguins (Eudyptes chrysolophus) in the Subantarctic Region. Appl. Environ. Microbiol. 66: 449-452 [Abstract] [Full Text]  
  • Lee, L. H., Burg, E. III, Baqar, S., Bourgeois, A. L., Burr, D. H., Ewing, C. P., Trust, T. J., Guerry, P. (1999). Evaluation of a Truncated Recombinant Flagellin Subunit Vaccine against Campylobacter jejuni. Infect. Immun. 67: 5799-5805 [Abstract] [Full Text]  
  • Duim, B., Wassenaar, T. M., Rigter, A., Wagenaar, J. (1999). High-Resolution Genotyping of Campylobacter Strains Isolated from Poultry and Humans with Amplified Fragment Length Polymorphism Fingerprinting. Appl. Environ. Microbiol. 65: 2369-2375 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»