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Journal of Clinical Microbiology, 11 1997, 2964-2965, Vol 35, No. 11
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Application of immunohistochemistry and in situ hybridization for detection of bovine coronavirus in paraffin-embedded, formalin-fixed intestines [In Process Citation]

Z Zhang, GA Andrews, C Chard-Bergstrom, HC Minocha and S Kapil
Department of Diagnostic Medicine-Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan 66506, USA.

A monoclonal antibody (MAb) (Z3A5) against spike protein subunit of bovine coronavirus (BCV) reacted with the virus in formalin-fixed intestines in an immunoperoxidase test. We found an 88% correlation between immunohistochemistry with Z3A5 and in situ hybridization with a BCV nucleoprotein cDNA probe. MAb Z3A5 reacted with 90 BCV isolates from the United States and was an effective reagent for the diagnosis of BCV.

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