This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Niemann, S.
Right arrow Articles by Richter, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Niemann, S.
Right arrow Articles by Richter, E.

Next Article 

Journal of Clinical Microbiology, Dec 1997, 3015-3020, Vol 35, No. 12
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

IS6110 fingerprinting of drug-resistant Mycobacterium tuberculosis strains isolated in Germany during 1995 [In Process Citation]

S Niemann, S Rusch-Gerdes and E Richter
Forschungszentrum Borstel, National Reference Center for Mycobacteria, Germany.

The epidemiological relatedness of drug-resistant Mycobacterium tuberculosis strains isolated in Germany in 1995 was evaluated by the standardized IS6110 fingerprinting method. Altogether, 196 M. tuberculosis isolates from 167 patients were analyzed. A large degree of IS6110 polymorphism was found, ranging from 1 to 20 copies. Multiple isolates from one patient generally remained stable over a period of up to 1 year. However, one strain showed an additional fragment 7 months after the first isolate was obtained. Isolates from 55 patients (33%) showed identical fingerprint patterns or fingerprint patterns that differed only in one band, and thus they were clustered in 22 fingerprint groups. Specific transmission links could be established between members of four groups, e.g., transmission by family contacts. In one case, transmission of a multidrug-resistant strain to a patient initially infected with a drug-susceptible strain could be shown. Besides these fingerprint groups, 30 of the 167 isolates (approximately 18%) could be grouped in two fingerprint clusters with a similarity of at least 78%. Approximately 60% of the patients of these two clusters were known to be immigrants from the former Soviet Union, and one patient is still living in Belarus. In conclusion, our results indicate that (i) transmission of drug-resistant strains contributes substantially to the emergence of drug-resistant tuberculosis in Germany and (ii) drug-resistant M. tuberculosis strains were presumably carried over from the former Soviet Union to Germany by immigrants.


This article has been cited by other articles:

  • Freidlin, P. J., Goldblatt, D., Kaidar-Shwartz, H., Rorman, E. (2009). Polymorphic Exact Tandem Repeat A (PETRA): a Newly Defined Lineage of Mycobacterium tuberculosis in Israel Originating Predominantly in Sub-Saharan Africa. J. Clin. Microbiol. 47: 4006-4020 [Abstract] [Full Text]  
  • Oelemann, M. C., Diel, R., Vatin, V., Haas, W., Rusch-Gerdes, S., Locht, C., Niemann, S., Supply, P. (2007). Assessment of an Optimized Mycobacterial Interspersed Repetitive- Unit-Variable-Number Tandem-Repeat Typing System Combined with Spoligotyping for Population-Based Molecular Epidemiology Studies of Tuberculosis. J. Clin. Microbiol. 45: 691-697 [Abstract] [Full Text]  
  • Kodmon, C., Niemann, S., Lukacs, J., Sor, E., David, S., Somoskovi, A. (2006). Molecular Epidemiology of Drug-Resistant Tuberculosis in Hungary. J. Clin. Microbiol. 44: 4258-4261 [Abstract] [Full Text]  
  • Gazouli, M., Ikonomopoulos, J., Koundourakis, A., Bartos, M., Pavlik, I., Overduin, P., Kremer, K., Gorgoulis, V., Kittas, C. (2005). Characterization of Mycobacterium tuberculosis Complex Isolates from Greek Patients with Sarcoidosis by Spoligotyping. J. Clin. Microbiol. 43: 4858-4861 [Abstract] [Full Text]  
  • Samper, S., Iglesias, M. J., Rabanaque, M. J., Gomez, L. I., Lafoz, M. C., Jimenez, M. S., Ortega, A., Lezcano, M. A, Van Soolingen, D., Martin, C., The Spanish Working Group on MDR-TB, (2005). Systematic Molecular Characterization of Multidrug-Resistant Mycobacterium tuberculosis Complex Isolates from Spain. J. Clin. Microbiol. 43: 1220-1227 [Abstract] [Full Text]  
  • Jou, R., Chiang, C.-Y., Huang, W.-L. (2005). Distribution of the Beijing Family Genotypes of Mycobacterium tuberculosis in Taiwan. J. Clin. Microbiol. 43: 95-100 [Abstract] [Full Text]  
  • Kam, K. M., Yip, C. W., Tse, L. W., Wong, K. L., Lam, T. K., Kremer, K., Au, B. K. Y., van Soolingen, D. (2005). Utility of Mycobacterial Interspersed Repetitive Unit Typing for Differentiating Multidrug-Resistant Mycobacterium tuberculosis Isolates of the Beijing Family. J. Clin. Microbiol. 43: 306-313 [Abstract] [Full Text]  
  • Lukacs, J., Tubak, V., Mester, J., David, S., Bartfai, Z., Kubica, T., Niemann, S., Somoskovi, A. (2004). Conventional and Molecular Epidemiology of Tuberculosis in Homeless Patients in Budapest, Hungary. J. Clin. Microbiol. 42: 5931-5934 [Abstract] [Full Text]  
  • Otsuka, Y., Parniewski, P., Zwolska, Z., Kai, M., Fujino, T., Kirikae, F., Toyota, E., Kudo, K., Kuratsuji, T., Kirikae, T. (2004). Characterization of a Trinucleotide Repeat Sequence (CGG)5 and Potential Use in Restriction Fragment Length Polymorphism Typing of Mycobacterium tuberculosis. J. Clin. Microbiol. 42: 3538-3548 [Abstract] [Full Text]  
  • Diel, R., Rusch-Gerdes, S., Niemann, S. (2004). Molecular Epidemiology of Tuberculosis among Immigrants in Hamburg, Germany. J. Clin. Microbiol. 42: 2952-2960 [Abstract] [Full Text]  
  • Seidler, A, Nienhaus, A, Diel, R (2004). The transmission of tuberculosis in the light of new molecular biological approaches. Occup. Environ. Med. 61: 96-102 [Abstract] [Full Text]  
  • Vukovic, D., Rusch-Gerdes, S., Savic, B., Niemann, S. (2003). Molecular Epidemiology of Pulmonary Tuberculosis in Belgrade, Central Serbia. J. Clin. Microbiol. 41: 4372-4377 [Abstract] [Full Text]  
  • Drobniewski, F. A., Gibson, A., Ruddy, M., Yates, M. D. (2003). Evaluation and Utilization as a Public Health Tool of a National Molecular Epidemiological Tuberculosis Outbreak Database within the United Kingdom from 1997 to 2001. J. Clin. Microbiol. 41: 1861-1868 [Abstract] [Full Text]  
  • Werngren, J., Hoffner, S. E. (2003). Drug-Susceptible Mycobacterium tuberculosis Beijing Genotype Does Not Develop Mutation-Conferred Resistance to Rifampin at an Elevated Rate. J. Clin. Microbiol. 41: 1520-1524 [Abstract] [Full Text]  
  • Puustinen, K., Marjamaki, M., Rastogi, N., Sola, C., Filliol, I., Ruutu, P., Holmstrom, P., Viljanen, M. K., Soini, H. (2003). Characterization of Finnish Mycobacterium tuberculosis Isolates by Spoligotyping. J. Clin. Microbiol. 41: 1525-1528 [Abstract] [Full Text]  
  • Diel, R., Schneider, S., Meywald-Walter, K., Ruf, C.-M., Rusch-Gerdes, S., Niemann, S. (2002). Epidemiology of Tuberculosis in Hamburg, Germany: Long-Term Population-Based Analysis Applying Classical and Molecular Epidemiological Techniques. J. Clin. Microbiol. 40: 532-539 [Abstract] [Full Text]  
  • Kruuner, A., Hoffner, S. E., Sillastu, H., Danilovits, M., Levina, K., Svenson, S. B., Ghebremichael, S., Koivula, T., Kallenius, G. (2001). Spread of Drug-Resistant Pulmonary Tuberculosis in Estonia. J. Clin. Microbiol. 39: 3339-3345 [Abstract] [Full Text]  
  • Antonucci, G., Girardi, E., Raviglione, M., Vanacore, P., Angarano, G., Chirianni, A., Pagano, G., Suter, F., Lauria, F.N., Ippolito, G., GISTA, (2001). Guidelines of tuberculosis preventive therapy for HIV-infected persons: a prospective, multicentre study. Eur Respir J 18: 369-375 [Abstract] [Full Text]  
  • Heep, M., Brandstätter, B., Rieger, U., Lehn, N., Richter, E., Rüsch-Gerdes, S., Niemann, S. (2001). Frequency of rpoB Mutations Inside and Outside the Cluster I Region in Rifampin-Resistant Clinical Mycobacterium tuberculosis Isolates. J. Clin. Microbiol. 39: 107-110 [Abstract] [Full Text]  
  • Niemann, S., Rüsch-Gerdes, S., Richter, E., Thielen, H., Heykes-Uden, H., Diel, R. (2000). Stability of IS6110 Restriction Fragment Length Polymorphism Patterns of Mycobacterium tuberculosis Strains in Actual Chains of Transmission. J. Clin. Microbiol. 38: 2563-2567 [Abstract] [Full Text]  
  • Gilad, J., Borer, A., Riesenberg, K., Peled, N., Schlaeffer, F. (2000). Epidemiology and Ethnic Distribution of Multidrug-Resistant Tuberculosis in Southern Israel, 1992-1997: The Impact of Immigration. Chest 117: 738-743 [Abstract] [Full Text]  
  • Fang, Z., Doig, C., Rayner, A., Kenna, D. T., Watt, B., Forbes, K. J. (1999). Molecular Evidence for Heterogeneity of the Multiple-Drug-Resistant Mycobacterium tuberculosis Population in Scotland (1990 to 1997). J. Clin. Microbiol. 37: 998-1003 [Abstract] [Full Text]  
  • Niemann, S., Richter, E., Rüsch-Gerdes, S. (1999). Stability of Mycobacterium tuberculosis IS6110 Restriction Fragment Length Polymorphism Patterns and Spoligotypes Determined by Analyzing Serial Isolates from Patients with Drug-Resistant Tuberculosis. J. Clin. Microbiol. 37: 409-412 [Abstract] [Full Text]