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Journal of Clinical Microbiology, Dec 1997, 3048-3053, Vol 35, No. 12
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Distinctive IS200 insertion between gyrA and rcsC genes in Salmonella typhi [In Process Citation]

E Calva, LG Ordonez, M Fernandez-Mora, FJ Santana, M Bobadilla and JL Puente
Departamento de Microbiologia Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos. ecalva@ibt.unam.mx

While probing the vicinity of ompC, a copy of the IS200 insertion element was found between the gyrA and rcsC genes of Salmonella typhi, the causal agent of typhoid fever. This distinctive feature was conserved throughout 63 S. typhi isolates of different geographical origins and was absent from 46 other Salmonella serotypes, including those most associated with human infections, as well as from 19 other enteric bacteria. Furthermore, the location of this IS200 copy corresponds to a constant band, present throughout the 14 PstI S. typhi IS200 fingerprints, encompassing several Vi phage types. Interestingly, an apparently unrelated serotype not frequently associated with human disease, Salmonella weltevreden, contained an IS200 copy at the same position, although it was accompanied by an additional segment of cryptic DNA. On the basis of these findings, PCR assays were designed for molecular typing of S. typhi, and these are potentially useful in studying the epidemiology of typhoid fever.


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Copyright © 1997 by the American Society for Microbiology. All rights reserved.