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Journal of Clinical Microbiology, Feb 1997, 446-449, Vol 35, No. 2
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

A new acid-fast trichrome stain for simultaneous detection of Cryptosporidium parvum and microsporidial species in stool specimens

R Ignatius, M Lehmann, K Miksits, T Regnath, M Arvand, E Engelmann, U Futh, H Hahn and J Wagner
Institut fur Medizinische Mikrobiologie und Infektionsimmunologie, Klinikum Benjamin Franklin der Freien Universitat Berlin, Germany.

The detection in stool specimens of Cryptosporidium parvum and microsporidia, the most frequent parasitic pathogens causing diarrhea in AIDS patients, until now has depended on two different staining methods. However, since double infections occur and minimization of laboratory costs is mandatory, development of a method for simultaneous detection of these parasites appeared desirable. We report on a new, inexpensive, and easy-to-perform staining procedure to demonstrate both acid-fast oocysts of C. parvum and other coccidia, as well as microsporidial spores. This acid-fast trichrome stain yields results comparable to those obtained by the Kinyoun and modified trichrome methods and considerably reduces the time necessary for microscopic examination.


This article has been cited by other articles:

  • Harrington, B. J. (2009). The Staining of Oocysts of Cryptosporidium With the Fluorescent Brighteners Uvitex 2B and Calcofluor White. Lab Med 40: 219-223 [Abstract] [Full Text]  
  • Nelson, S. P., Lin, P. L., Miller, J., Katz, B. Z., Gonzalez-Crussi, F. (1999). Cryptosporidia Enterocolitis in an Immunocompetent Infant Treated with Paromomycin. CLIN PEDIATR 38: 367-369  


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