This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Delaney, M. L. Articles by Onderdonk, A. B. Search for Related Content PubMed PubMed Citation Articles by Delaney, M. L. Articles by Onderdonk, A. B.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 03 1997, 558-562, Vol 35, No. 3
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Evaluation of the AnaeroPack system for growth of clinically significant anaerobes

ML Delaney and AB Onderdonk
Brigham & Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA. mldelaney@bics.bwh.harvard.edu

The AnaeroPack (Mitsubishi Gas Chemical America, Inc., New York, N.Y.) system was compared with the GasPak (Becton Dickinson Microbiology Systems, Cockeysville, Md.) system and a conventional anaerobe chamber to evaluate the ability of the AnaeroPack system to support the growth of clinically significant anaerobes. The AnaeroPack system requires no catalyst or water, produces no hydrogen, and is oxygen absorbing and carbon dioxide generating. It is simple to use and reduces preparation time to a minimum. One hundred forty clinical isolates obtained from various anatomic sites and 10 American Type Culture Collection type strains were evaluated. Isolates were plated on various media, and bacterial growth was examined after 24, 48, 72, and 168 h of incubation. Criteria for evaluation and comparison of systems included rate and quality of growth, colonial morphology, hemolytic reactions, and pigment production. Results indicate that the AnaeroPack system is highly effective in creating an anaerobic atmosphere. The AnaeroPack system never failed to reduce the methylene blue indicator, while the GasPak system failed 15% of the time. The rate or quality of growth achieved by the AnaeroPack system compared with that of established anaerobic culturing techniques was similar and significantly better for several genera including the Bacteroides fragilis group, Fusobacterium, Clostridium, and Peptostreptococcus. The AnaeroPack system appears to be an excellent alternative to established methods for generating an environment for anaerobic incubation.

This article has been cited by other articles:

• Sato, H., Sato, M., Kanai, H., Uchiyama, T., Iso, T., Ohyama, Y., Sakamoto, H., Tamura, J., Nagai, R., Kurabayashi, M. (2005). Mitochondrial reactive oxygen species and c-Src play a critical role in hypoxic response in vascular smooth muscle cells. Cardiovasc Res 67: 714-722 [Abstract] [Full Text]  
• Sato, M., Tanaka, T., Maeno, T., Sando, Y., Suga, T., Maeno, Y., Sato, H., Nagai, R., Kurabayashi, M. (2002). Inducible Expression of Endothelial PAS Domain Protein-1 by Hypoxia in Human Lung Adenocarcinoma A549 Cells . Role of Src Family Kinases-dependent Pathway. Am. J. Respir. Cell Mol. Bio. 26: 127-134 [Abstract] [Full Text]  
• Uchiyama, T., Kurabayashi, M., Ohyama, Y., Utsugi, T., Akuzawa, N., Sato, M., Tomono, S., Kawazu, S., Nagai, R. (2000). Hypoxia Induces Transcription of the Plasminogen Activator Inhibitor-1 Gene Through Genistein-Sensitive Tyrosine Kinase Pathways in Vascular Endothelial Cells. Arterioscler. Thromb. Vasc. Bio. 20: 1155-1161 [Abstract] [Full Text]  
• Van Horn, K., Tóth, C. (1999). Evaluation of the AnaeroPack Campylo System for Growth of Microaerophilic Bacteria. J. Clin. Microbiol. 37: 2376-2377 [Abstract] [Full Text]  
• Doan, N., Contreras, A., Flynn, J., Morrison, J., Slots, J. (1999). Proficiencies of Three Anaerobic Culture Systems for Recovering Periodontal Pathogenic Bacteria. J. Clin. Microbiol. 37: 171-174 [Abstract] [Full Text]