This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sander, A. Articles by Bredt, W. Search for Related Content PubMed PubMed Citation Articles by Sander, A. Articles by Bredt, W.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, Mar 1997, 584-587, Vol 35, No. 3
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Detection and identification of two Bartonella henselae variants in domestic cats in Germany

A Sander, C Buhler, K Pelz, E von Cramm and W Bredt
Abteilung Mikrobiologie und Hygiene, Klinikum der Universitat Freiburg, Germany.

To determine the prevalence of bacteremia caused by Bartonella henselae in domestic cats in the region of Freiburg, Germany, we investigated culture of blood from 100 cats from 89 different households over a 12- month period. B. henselae could be isolated from 13% (13 of 100) of these cats. In eight households with two cats each and in one household with three cats, B. henselae bacteremia was found either in all of the animals or in none of the animals. Positive cultures were more likely to be found for female, young (24 months of age or younger) cats than for male or older cats. Identification of the Bartonella isolates was made by colony morphology, by Gram staining, biochemically by RapID ANA II or Rapid ID 32 A systems, and by whole-cell fatty acid analysis. Differentiation between B. henselae and Bartonella quintana was only possible by 16S rRNA sequencing, enterobacterial repetitive intergenic consensus (ERIC)-PCR and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Genomic fingerprinting of the B. henselae isolates by ERIC-PCR yielded two different patterns based on three distinct bands.

This article has been cited by other articles:

• Viezens, J., Arvand, M. (2008). Simultaneous presence of two different copies of the 16S rRNA gene in Bartonella henselae. Microbiology 154: 2881-2886 [Abstract] [Full Text]  
• Ridder-Schroter, R., Marx, A., Beer, M., Tappe, D., Kreth, H.-W., Girschick, H. J. (2008). Abscess-forming lymphadenopathy and osteomyelitis in children with Bartonella henselae infection. J Med Microbiol 57: 519-524 [Abstract] [Full Text]  
• Riess, T., Raddatz, G., Linke, D., Schafer, A., Kempf, V. A. J. (2007). Analysis of Bartonella Adhesin A Expression Reveals Differences between Various B. henselae Strains. Infect. Immun. 75: 35-43 [Abstract] [Full Text]  
• Dorbecker, C., Sander, A., Oberle, K., Schulin-Casonato, T. (2006). In vitro susceptibility of Bartonella species to 17 antimicrobial compounds: comparison of Etest and agar dilution. J Antimicrob Chemother 58: 784-788 [Abstract] [Full Text]  
• PONS, I., SANFELIU, I., QUESADA, M., ANTON, E., SAMPERE, M., FONT, B., PLA, J., SEGURA, F. (2005). PREVALENCE OF BARTONELLA HENSELAE IN CATS IN CATALONIA, SPAIN. Am J Trop Med Hyg 72: 453-457 [Abstract] [Full Text]  
• Guptill, L., Wu, C.-C., HogenEsch, H., Slater, L. N., Glickman, N., Dunham, A., Syme, H., Glickman, L. (2004). Prevalence, Risk Factors, and Genetic Diversity of Bartonella henselae Infections in Pet Cats in Four Regions of the United States. J. Clin. Microbiol. 42: 652-659 [Abstract] [Full Text]  
• Fabbi, M., De Giuli, L., Tranquillo, M., Bragoni, R., Casiraghi, M., Genchi, C. (2004). Prevalence of Bartonella henselae in Italian Stray Cats: Evaluation of Serology To Assess the Risk of Transmission of Bartonella to Humans. J. Clin. Microbiol. 42: 264-268 [Abstract] [Full Text]  
• Zimmermann, R., Kempf, V. A. J., Schiltz, E., Oberle, K., Sander, A. (2003). Hemin Binding, Functional Expression, and Complementation Analysis of Pap 31 from Bartonella henselae. J. Bacteriol. 185: 1739-1744 [Abstract] [Full Text]  
• Dillon, B., Valenzuela, J., Don, R., Blanckenberg, D., Wigney, D. I., Malik, R., Morris, A. J., Robson, J. M., Iredell, J. (2002). Limited Diversity among Human Isolates of Bartonella henselae. J. Clin. Microbiol. 40: 4691-4699 [Abstract] [Full Text]  
• Fournier, P.-E., Robson, J., Zeaiter, Z., McDougall, R., Byrne, S., Raoult, D. (2002). Improved Culture from Lymph Nodes of Patients with Cat Scratch Disease and Genotypic Characterization of Bartonella henselae Isolates in Australia. J. Clin. Microbiol. 40: 3620-3624 [Abstract] [Full Text]  
• Zeaiter, Z., Liang, Z., Raoult, D. (2002). Genetic Classification and Differentiation of Bartonella Species Based on Comparison of Partial ftsZ Gene Sequences. J. Clin. Microbiol. 40: 3641-3647 [Abstract] [Full Text]  
• La Scola, B., Liang, Z., Zeaiter, Z., Houpikian, P., Grimont, P. A. D., Raoult, D. (2002). Genotypic Characteristics of Two Serotypes of Bartonella henselae. J. Clin. Microbiol. 40: 2002-2008 [Abstract] [Full Text]  
• Zeaiter, Z., Fournier, P.-E., Raoult, D. (2002). Genomic Variation of Bartonella henselae Strains Detected in Lymph Nodes of Patients with Cat Scratch Disease. J. Clin. Microbiol. 40: 1023-1030 [Abstract] [Full Text]  
• Yamamoto, K., Chomel, B. B., Kasten, R. W., Hew, C. M., Weber, D. K., Lee, W. I., Droz, S., Koehler, J. E. (2002). Experimental Infection of Domestic Cats with Bartonella koehlerae and Comparison of Protein and DNA Profiles with Those of Other Bartonella Species Infecting Felines. J. Clin. Microbiol. 40: 466-474 [Abstract] [Full Text]  
• Arvand, M., Klose, A. J., Schwartz-Porsche, D., Hahn, H., Wendt, C. (2001). Genetic Variability and Prevalence of Bartonella henselae in Cats in Berlin, Germany, and Analysis of Its Genetic Relatedness to a Strain from Berlin That Is Pathogenic for Humans. J. Clin. Microbiol. 39: 743-746 [Abstract] [Full Text]  
• Chang, C.-C., Kasten, R. W., Chomel, B. B., Simpson, D. C., Hew, C. M., Kordick, D. L., Heller, R., Piemont, Y., Breitschwerdt, E. B. (2000). Coyotes (Canis latrans) as the Reservoir for a Human Pathogenic Bartonella sp.: Molecular Epidemiology of Bartonella vinsonii subsp. berkhoffii Infection in Coyotes from Central Coastal California. J. Clin. Microbiol. 38: 4193-4200 [Abstract] [Full Text]  
• Sander, A., Zagrosek, A., Bredt, W., Schiltz, E., Piémont, Y., Lanz, C., Dehio, C. (2000). Characterization of Bartonella clarridgeiae Flagellin (FlaA) and Detection of Antiflagellin Antibodies in Patients with Lymphadenopathy. J. Clin. Microbiol. 38: 2943-2948 [Abstract] [Full Text]  
• Handley, S. A., Regnery, R. L. (2000). Differentiation of Pathogenic Bartonella Species by Infrequent Restriction Site PCR. J. Clin. Microbiol. 38: 3010-3015 [Abstract] [Full Text]  
• Breitschwerdt, E. B., Kordick, D. L. (2000). Bartonella Infection in Animals: Carriership, Reservoir Potential, Pathogenicity, and Zoonotic Potential for Human Infection. Clin. Microbiol. Rev. 13: 428-438 [Abstract] [Full Text]  
• Ehrenborg, C., Wesslén, L., Jakobson, A., Friman, G., Holmberg, M. (2000). Sequence Variation in the ftsZ Gene of Bartonella henselae Isolates and Clinical Samples. J. Clin. Microbiol. 38: 682-687 [Abstract] [Full Text]  
• Sander, A., Penno, S. (1999). Semiquantitative Species-Specific Detection of Bartonella henselae and Bartonella quintana by PCR-Enzyme Immunoassay. J. Clin. Microbiol. 37: 3097-3101 [Abstract] [Full Text]  
• Kordick, D. L., Brown, T. T., Shin, K., Breitschwerdt, E. B. (1999). Clinical and Pathologic Evaluation of Chronic Bartonella henselae or Bartonella clarridgeiae Infection in Cats. J. Clin. Microbiol. 37: 1536-1547 [Abstract] [Full Text]  
• Sander, A., Posselt, M., Böhm, N., Ruess, M., Altwegg, M. (1999). Detection of Bartonella henselae DNA by Two Different PCR Assays and Determination of the Genotypes of Strains Involved in Histologically Defined Cat Scratch Disease. J. Clin. Microbiol. 37: 993-997 [Abstract] [Full Text]  
• Marston, E. L., Finkel, B., Regnery, R. L., Winoto, I. L., Graham, R. R., Wignal, S., Simanjuntak, G., Olson, J. G. (1999). Prevalence of Bartonella henselae and Bartonella clarridgeiae in an Urban Indonesian Cat Population. CVI 6: 41-44 [Abstract] [Full Text]  
• Sander, A., Ruess, M., Bereswill, S., Schuppler, M., Steinbrueckner, B. (1998). Comparison of Different DNA Fingerprinting Techniques for Molecular Typing of Bartonella henselae Isolates. J. Clin. Microbiol. 36: 2973-2981 [Abstract] [Full Text]  
• Sander, A., Posselt, M., Oberle, K., Bredt, W. (1998). Seroprevalence of Antibodies to Bartonella henselae in Patients with Cat Scratch Disease and in Healthy Controls: Evaluation and Comparison of Two Commercial Serological Tests. CVI 5: 486-490 [Abstract] [Full Text]