This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gamble, D. A. Articles by Colucci, G. Search for Related Content PubMed PubMed Citation Articles by Gamble, D. A. Articles by Colucci, G.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 03 1997, 673-675, Vol 35, No. 3
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Development of a nested PCR assay for detection of feline infectious peritonitis virus in clinical specimens

DA Gamble, A Lobbiani, M Gramegna, LE Moore and G Colucci
Animal Medical Center, New York, New York, USA.

A diagnostic test for feline infectious peritonitis virus (FIPV) infection based on a nested PCR (nPCR) assay was developed and tested with FIPV, feline enteric coronavirus (FECV), canine coronavirus (CCV), and transmissible gastroenteritis virus (TGEV) and clinical fluid samples from cats with effusive feline infectious peritonitis (FIP). The target sequence for the assay is in the S1 region of the peplomer protein E2 gene. A vaccine strain of FIPV and two wild-type FIPV strains tested positive, but FECV, TGEV, and CCV tested negative. Preliminary tests with 12 cats with clinical evidence of effusive FIP and 11 cats with an illness associated with effusions, but attributed to other causes, were performed. Eleven of the 12 cats with effusive FIP tested positive, while 1 was negative. Ten of the 11 cats ill from other causes tested negative, while 1 was positive. On the basis of clinical laboratory and histopathologic criteria, the preliminary sensitivity and specificity of the assay were 91.6 and 94%, respectively.

This article has been cited by other articles:

• Naylor, M. J., Harrison, G. A., Monckton, R. P., McOrist, S., Lehrbach, P. R., Deane, E. M. (2001). Identification of Canine Coronavirus Strains from Feces by S Gene Nested PCR and Molecular Characterization of a New Australian Isolate. J. Clin. Microbiol. 39: 1036-1041 [Abstract] [Full Text]  
• Frisk, A. L., König, M., Moritz, A., Baumgärtner, W. (1999). Detection of Canine Distemper Virus Nucleoprotein RNA by Reverse Transcription-PCR Using Serum, Whole Blood, and Cerebrospinal Fluid from Dogs with Distemper. J. Clin. Microbiol. 37: 3634-3643 [Abstract] [Full Text]