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Journal of Clinical Microbiology, Apr 1997, 976-977, Vol 35, No. 4
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Rapid diagnosis of enterovirus infection by a new one-step reverse transcription-PCR assay

HH Kessler, B Santner, H Rabenau, A Berger, A Vince, C Lewinski, B Weber, K Pierer, D Stuenzner, E Marth and HW Doerr
Institute of Hygiene, KF-University Graz, Austria. harald.kessler@kfunigraz.ac.at

The AMPLICOR Enterovirus Test was evaluated with 103 cerebrospinal fluid (CSF) specimens. Twenty-seven CSF specimens were culture positive. With the AMPLICOR test, enterovirus RNA was detected in 34 specimens. Compared with culture, the AMPLICOR test gave a sensitivity of 96.3% and a specificity of 100%. The sensitivity of culture was 79.4% in comparison with the AMPLICOR test.

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