This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chernesky, M. A. Articles by Mahony, J. B. Search for Related Content PubMed PubMed Citation Articles by Chernesky, M. A. Articles by Mahony, J. B.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, Apr 1997, 982-984, Vol 35, No. 4
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Ability of commercial ligase chain reaction and PCR assays to diagnose Chlamydia trachomatis infections in men by testing first-void urine

MA Chernesky, S Chong, D Jang, K Luinstra, J Sellors and JB Mahony
McMaster University Regional Virology and Chlamydiology Laboratory, St. Joseph's Hospital, Hamilton, Ontario, Canada.

A total of 287 men (37.6% with symptoms of urethritis) attending a hospital-based sexually transmitted disease clinic had urethral swabs tested by culture and by direct fluorescent-antibody assay. First-void urine (FVU) was tested for Chlamydia trachomatis by commercially available ligase chain reaction (LCR) and PCR assays. By using an expanded reference standard, 35 men (12.2%) were found to be positive. By performing LCR and PCR, the infection prevalence was found to be approximately twice (11.5 and 12.2%, respectively) that determined swab testing. The sensitivity values were 94.3% for LCR and 100% for PCR. One of the two positive specimens missed by LCR contained inhibitors. PCR produced five false-positive results and LCR produced one.

This article has been cited by other articles:

• Chernesky, M. A., Martin, D. H., Hook, E. W., Willis, D., Jordan, J., Wang, S., Lane, J. R., Fuller, D., Schachter, J. (2005). Ability of New APTIMA CT and APTIMA GC Assays To Detect Chlamydia trachomatis and Neisseria gonorrhoeae in Male Urine and Urethral Swabs. J. Clin. Microbiol. 43: 127-131 [Abstract] [Full Text]  
• Dalesio, N., Marsiglia, V., Quinn, A., Quinn, T. C., Gaydos, C. A. (2004). Performance of the MagNA Pure LC Robot for Extraction of Chlamydia trachomatis and Neisseria gonorrhoeae DNA from Urine and Swab Specimens. J. Clin. Microbiol. 42: 3300-3302 [Abstract] [Full Text]  
• Black, C. M., Marrazzo, J., Johnson, R. E., Hook III, E. W., Jones, R. B., Green, T. A., Schachter, J., Stamm, W. E., Bolan, G., St. Louis, M. E., Martin, D. H. (2002). Head-to-Head Multicenter Comparison of DNA Probe and Nucleic Acid Amplification Tests for Chlamydia trachomatis Infection in Women Performed with an Improved Reference Standard. J. Clin. Microbiol. 40: 3757-3763 [Abstract] [Full Text]  
• Land, S., Tabrizi, S., Gust, A., Johnson, E., Garland, S., Dax, E. M. (2002). External Quality Assessment Program for Chlamydia trachomatis Diagnostic Testing by Nucleic Acid Amplification Assays. J. Clin. Microbiol. 40: 2893-2896 [Abstract] [Full Text]  
• Mahony, J. B., Song, X., Chong, S., Faught, M., Salonga, T., Kapala, J. (2001). Evaluation of the NucliSens Basic Kit for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in Genital Tract Specimens Using Nucleic Acid Sequence-Based Amplification of 16S rRNA. J. Clin. Microbiol. 39: 1429-1435 [Abstract] [Full Text]  
• van der Paardt, M, van Denderen, J C, van den Brule, A J C, Morré, S A, van der Horst-Bruinsma, I E, Bezemer, P D, Dijkmans, B A C (2000). Prevalence of Chlamydia trachomatis in urine of male patients with ankylosing spondylitis is not increased. Ann Rheum Dis 59: 300-302 [Abstract] [Full Text]  
• Van Der Pol, B., Quinn, T. C., Gaydos, C. A., Crotchfelt, K., Schachter, J., Moncada, J., Jungkind, D., Martin, D. H., Turner, B., Peyton, C., Jones, R. B. (2000). Multicenter Evaluation of the AMPLICOR and Automated COBAS AMPLICOR CT/NG Tests for Detection of Chlamydia trachomatis. J. Clin. Microbiol. 38: 1105-1112 [Abstract] [Full Text]  
• Morré, S. A., Van Valkengoed, I. G. M., Moes, R. M., Boeke, A. J. P., Meijer, C. J. L. M., Van den Brule, A. J. C. (1999). Determination of Chlamydia trachomatis Prevalence in an Asymptomatic Screening Population: Performances of the LCx and COBAS Amplicor Tests with Urine Specimens. J. Clin. Microbiol. 37: 3092-3096 [Abstract] [Full Text]  
• Vincelette, J., Schirm, J., Bogard, M., Bourgault, A.-M., Luijt, D. S., Bianchi, A., van Voorst Vader, P. C., Butcher, A., Rosenstraus, M. (1999). Multicenter Evaluation of the Fully Automated COBAS AMPLICOR PCR Test for Detection of Chlamydia trachomatis in Urogenital Specimens. J. Clin. Microbiol. 37: 74-80 [Abstract] [Full Text]  
• Wylie, J. L., Moses, S., Babcock, R., Jolly, A., Giercke, S., Hammond, G. (1998). Comparative Evaluation of Chlamydiazyme, PACE 2, and AMP-CT Assays for Detection of Chlamydia trachomatis in Endocervical Specimens. J. Clin. Microbiol. 36: 3488-3491 [Abstract] [Full Text]  
• Toye, B., Woods, W., Bobrowska, M., Ramotar, K. (1998). Inhibition of PCR in Genital and Urine Specimens Submitted for Chlamydia trachomatis Testing. J. Clin. Microbiol. 36: 2356-2358 [Abstract] [Full Text]  
• Puolakkainen, M., Hiltunen-Back, E., Reunala, T., Suhonen, S., Lähteenmäki, P., Lehtinen, M., Paavonen, J. (1998). Comparison of Performances of Two Commercially Available Tests, a PCR Assay and a Ligase Chain Reaction Test, in Detection of Urogenital Chlamydia trachomatis Infection. J. Clin. Microbiol. 36: 1489-1493 [Abstract] [Full Text]  
• Kacena, K. A., Quinn, S. B., Howell, M. R., Madico, G. E., Quinn, T. C., Gaydos, C. A. (1998). Pooling Urine Samples for Ligase Chain Reaction Screening for Genital Chlamydia trachomatis Infection in Asymptomatic Women. J. Clin. Microbiol. 36: 481-485 [Abstract] [Full Text]  
• Rosenstraus, M, Wang, Z, Chang, S., DeBonville, D, Spadoro, J. (1998). An internal control for routine diagnostic PCR: design, properties, and effect on clinical performance [In Process Citation]. J. Clin. Microbiol. 36: 191-197 [Abstract] [Full Text]