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Journal of Clinical Microbiology, Apr 1997, 995-998, Vol 35, No. 4
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model

L Monteiro, D Bonnemaison, A Vekris, KG Petry, J Bonnet, R Vidal, J Cabrita and F Megraud
Laboratoire de Bacteriologie, Universite de Bordeaux 2, France. helico@labhel.u-bordeaux2.fr

A model was developed to study inhibitors present in feces which prevent the use of PCR for the detection of Helicobacter pylori. A DNA fragment amplified with the same primers as H. pylori was used to spike samples before extraction by a modified QIAamp tissue method. Inhibitors, separated on an Ultrogel AcA44 column, were characterized. Inhibitors in feces are complex polysaccharides possibly originating from vegetable material in the diet.

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