This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Izumiya, H. Articles by Watanabe, H. Search for Related Content PubMed PubMed Citation Articles by Izumiya, H. Articles by Watanabe, H.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 07 1997, 1675-1680, Vol 35, No. 7
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Molecular typing of enterohemorrhagic Escherichia coli O157:H7 isolates in Japan by using pulsed-field gel electrophoresis

H Izumiya, J Terajima, A Wada, Y Inagaki, KI Itoh, K Tamura and H Watanabe
Department of Bacteriology, National Institute of Health, Shinjuku, Tokyo, Japan.

Pulsed-field gel electrophoresis (PFGE) was applied for molecular typing of 825 enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates, most of which were from 19 outbreaks and 608 sporadic cases in Japan, mainly in May to August 1996. By PFGE, the EHEC O157:H7 isolates were classified into six types (type I to V and ND [nondescript]) and UT untypeable isolates. Fifty isolates from seven outbreaks in May to June and 60 isolates from patients with sporadic cases of infection showed almost identical PFGE patterns which differed in only 1 of 22 DNA fragments. They were classified into type I. Ninety- nine isolates from 10 other outbreaks and 156 isolates from patients in the Kinki area with sporadic cases of infection obtained in the early summer of 1996 showed identical PFGE patterns, suggesting that they were derived from one huge outbreak. They were classified into type II. Type IV EHEC isolates, which had only the stx2 gene, caused another outbreak in a primary school in June. EHEC isolates of two other types, types III and V, were not related to the outbreak but were isolated in several parts of Japan. ND EHEC isolates included a variety of patterns which could not be classified into either of the types mentioned above. Twenty-five isolates could not be analyzed due to degradation of their genomic DNAs and were represented as UT. These results indicate that EHEC O157:H7 strains with various PFGE types have already spread to Japan and caused the multiple outbreaks and sporadic infections in Japan in the summer of 1996.

This article has been cited by other articles:

• Kawamori, F., Hiroi, M., Harada, T., Ohata, K., Sugiyama, K., Masuda, T., Ohashi, N. (2008). Molecular typing of Japanese Escherichia coli O157 : H7 isolates from clinical specimens by multilocus variable-number tandem repeat analysis and PFGE. J Med Microbiol 57: 58-63 [Abstract] [Full Text]  
• Kalpoe, J. S., Templeton, K. E., Horrevorts, A. M., Endtz, H. P., Kuijper, E. J., Bernards, A. T., Klaassen, C. H. W. (2007). Molecular Typing of a Suspected Cluster of Nocardia farcinica Infections by Use of Randomly Amplified Polymorphic DNA, Pulsed-Field Gel Electrophoresis, and Amplified Fragment Length Polymorphism Analyses. J. Clin. Microbiol. 45: 4048-4050 [Abstract] [Full Text]  
• Vaz, T. M. I., Irino, K., Nishimura, L. S., Cergole-Novella, M. C., Guth, B. E. C. (2006). Genetic Heterogeneity of Shiga Toxin-Producing Escherichia coli Strains Isolated in Sao Paulo, Brazil, from 1976 through 2003, as Revealed by Pulsed-Field Gel Electrophoresis.. J. Clin. Microbiol. 44: 798-804 [Abstract] [Full Text]  
• Ohtsuka, K., Yanagawa, K., Takatori, K., Hara-Kudo, Y. (2005). Detection of Salmonella enterica in Naturally Contaminated Liquid Eggs by Loop-Mediated Isothermal Amplification, and Characterization of Salmonella Isolates. Appl. Environ. Microbiol. 71: 6730-6735 [Abstract] [Full Text]  
• Bidet, P., Mariani-Kurkdjian, P., Grimont, F., Brahimi, N., Courroux, C., Grimont, P., Bingen, E. (2005). Characterization of Escherichia coli O157 : H7 isolates causing haemolytic uraemic syndrome in France. J Med Microbiol 54: 71-75 [Abstract] [Full Text]  
• Suzuki, M., Matsumoto, M., Hata, M., Takahashi, M., Sakae, K. (2004). Development of a Rapid PCR Method Using the Insertion Sequence IS1203 for Genotyping Shiga Toxin-Producing Escherichia coli O157. J. Clin. Microbiol. 42: 5462-5466 [Abstract] [Full Text]  
• Singer, R. S., Sischo, W. M., Carpenter, T. E. (2004). Exploration of Biases That Affect the Interpretation of Restriction Fragment Patterns Produced by Pulsed-Field Gel Electrophoresis. J. Clin. Microbiol. 42: 5502-5511 [Abstract] [Full Text]  
• Shima, K., Terajima, J., Sato, T., Nishimura, K., Tamura, K., Watanabe, H., Takeda, Y., Yamasaki, S. (2004). Development of a PCR-Restriction Fragment Length Polymorphism Assay for the Epidemiological Analysis of Shiga Toxin-Producing Escherichia coli. J. Clin. Microbiol. 42: 5205-5213 [Abstract] [Full Text]  
• LeJeune, J. T., Besser, T. E., Rice, D. H., Berg, J. L., Stilborn, R. P., Hancock, D. D. (2004). Longitudinal Study of Fecal Shedding of Escherichia coli O157:H7 in Feedlot Cattle: Predominance and Persistence of Specific Clonal Types despite Massive Cattle Population Turnover. Appl. Environ. Microbiol. 70: 377-384 [Abstract] [Full Text]  
• Davis, M. A., Hancock, D. D., Besser, T. E., Call, D. R. (2003). Evaluation of Pulsed-Field Gel Electrophoresis as a Tool for Determining the Degree of Genetic Relatedness between Strains of Escherichia coli O157:H7. J. Clin. Microbiol. 41: 1843-1849 [Abstract] [Full Text]  
• Noller, A. C., McEllistrem, M. C., Stine, O. C., Morris, J. G. Jr., Boxrud, D. J., Dixon, B., Harrison, L. H. (2003). Multilocus Sequence Typing Reveals a Lack of Diversity among Escherichia coli O157:H7 Isolates That Are Distinct by Pulsed-Field Gel Electrophoresis. J. Clin. Microbiol. 41: 675-679 [Abstract] [Full Text]  
• Giammanco, G. M., Pignato, S., Grimont, F., Grimont, P. A. D., Caprioli, A., Morabito, S., Giammanco, G. (2002). Characterization of Shiga Toxin-Producing Escherichia coli O157:H7 Isolated in Italy and in France. J. Clin. Microbiol. 40: 4619-4624 [Abstract] [Full Text]  
• Iguchi, A., Osawa, R., Kawano, J., Shimizu, A., Terajima, J., Watanabe, H. (2002). Effects of Repeated Subculturing and Prolonged Storage at Room Temperature of Enterohemorrhagic Escherichia coli O157:H7 on Pulsed-Field Gel Electrophoresis Profiles. J. Clin. Microbiol. 40: 3079-3081 [Abstract] [Full Text]  
• NAGANO, H., OKUI, T., FUJIWARA, O., UCHIYAMA, Y., TAMATE, N., KUMADA, H., MORIMOTO, Y., YANO, S. (2002). Clonal structure of Shiga toxin (Stx)-producing and {beta}-D-glucuronidase-positive Escherichia coli O157:H7 strains isolated from outbreaks and sporadic cases in Hokkaido, Japan. J Med Microbiol 51: 405-416 [Abstract] [Full Text]  
• Klaassen, C. H. W., van Haren, H. A., Horrevorts, A. M. (2002). Molecular Fingerprinting of Clostridium difficile Isolates: Pulsed-Field Gel Electrophoresis versus Amplified Fragment Length Polymorphism. J. Clin. Microbiol. 40: 101-104 [Abstract] [Full Text]  
• Izumiya, H., Terajima, J., Matsushita, S., Tamura, K., Watanabe, H. (2001). Characterization of Multidrug-Resistant Salmonella enterica Serovar Typhimurium Isolated in Japan. J. Clin. Microbiol. 39: 2700-2703 [Abstract] [Full Text]  
• Kopytek, S. J., Dyer, J. C. D., Knapp, G. S., Hu, J. C. (2000). Resistance to Methotrexate Due to AcrAB-Dependent Export from Escherichia coli. Antimicrob. Agents Chemother. 44: 3210-3212 [Abstract] [Full Text]  
• OSAWA, R., IYODA, S., NAKAYAMA, S.-I., WADA, A., YAMAI, S., WATANABE, H. (2000). Genotypic variations of Shiga toxin-converting phages from enterohaemorrhagic Escherichia coli O157: H7 isolates. J Med Microbiol 49: 565-574 [Abstract] [Full Text]  
• Allison, L. J., Carter, P. E., Thomson-Carter, F. M. (2000). Characterization of a Recurrent Clonal Type of Escherichia coli O157:H7 Causing Major Outbreaks of Infection in Scotland. J. Clin. Microbiol. 38: 1632-1635 [Abstract] [Full Text]  
• Arakawa, E., Murase, T., Matsushita, S., Shimada, T., Yamai, S., Ito, T., Watanabe, H. (2000). Pulsed-Field Gel Electrophoresis-Based Molecular Comparison of Vibrio cholerae O1 Isolates from Domestic and Imported Cases of Cholera in Japan. J. Clin. Microbiol. 38: 424-426 [Abstract] [Full Text]  
• Mitsuda, T., Kuroki, H., Ishikawa, N., Imagawa, T., Ito, S., Miyamae, T., Mori, M., Uehara, S., Yokota, S. (1999). Molecular Epidemiological Study of Haemophilus influenzae Serotype b Strains Obtained from Children with Meningitis in Japan. J. Clin. Microbiol. 37: 2548-2552 [Abstract] [Full Text]  
• Reid, S. D., Betting, D. J., Whittam, T. S. (1999). Molecular Detection and Identification of Intimin Alleles in Pathogenic Escherichia coli by Multiplex PCR. J. Clin. Microbiol. 37: 2719-2722 [Abstract] [Full Text]  
• Keskimäki, M., Saari, M., Heiskanen, T., Siitonen, A. (1998). Shiga Toxin-Producing Escherichia coli in Finland from 1990 through 1997: Prevalence and Characteristics of Isolates. J. Clin. Microbiol. 36: 3641-3646 [Abstract] [Full Text]