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Journal of Clinical Microbiology, Aug 1997, 2133-2135, Vol 35, No. 8
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Diagnosis of genitourinary Chlamydia trachomatis infections by using the ligase chain reaction on patient-obtained vaginal swabs

EW Hook 3rd, K Smith, C Mullen, J Stephens, L Rinehardt, MS Pate and HH Lee
University of Alabama at Birmingham and Jefferson County Department of Health, 35294-0006, USA. ehook@uabid.dom.uab.edu

We compared the ligase chain reaction (LCR) assay to cell culture for diagnosis of genitourinary chlamydial infections in women using swab specimens obtained by clinicians from the endocervix and by patients from their own vaginas. Specimens from 40 (12.9%) of 309 patients were positive for chlamydial infection by culture, while the specimens of 50 (16.2%) patients were positive by LCR. Chlamydia trachomatis infection was verified for 9 of 10 patients whose LCR specimens were positive but whose cultures were negative. Vaginal and cervical swab specimens were positive by LCR for 46 (93.9%) and 44 (89.8%) of 49 chlamydia-infected patients, respectively. These data suggest that LCR testing for chlamydia with vaginal swab specimens obtained by patients themselves is as sensitive as cervical LCR and more sensitive than cell culture.

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