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Journal of Clinical Microbiology, Sep 1997, 2337-2341, Vol 35, No. 9
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Identification of clinically relevant viridans group streptococci to the species level by PCR

F Garnier, G Gerbaud, P Courvalin and M Galimand
Unite des Agents Antibacteriens and National Reference Center for Antibiotics, Institut Pasteur, Paris, France.

A PCR assay that allows identification of clinically relevant viridans group streptococci (Streptococcus gordonii, S. mitis, S. mutans, S. oralis, S. salivarius, and S. sanguis) to the species level and identification of milleri group streptococci (S. anginosus, S. constellatus, and S. intermedius) to the group level was developed. This assay was based on specific amplification of internal fragments of genes encoding D-alanine:D-alanine ligases which are species specific and ubiquitous in prokaryotes possessing peptidoglycan. The specificity of this assay was tested on 9 reference strains and 91 characterized clinical isolates. This assay offers a specific and rapid alternative to phenotypic or DNA-DNA hybridization methods for identification of clinically relevant viridans group streptococci.

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