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Journal of Clinical Microbiology, 09 1997, 2401-2404, Vol 35, No. 9
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

A molecular scheme based on 23S rRNA gene polymorphisms for rapid identification of Campylobacter and Arcobacter species

A Hurtado and RJ Owen
Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, United Kingdom.

23S rRNA gene PCR amplicons from 118 strains representing 15 species of Campylobacter and four species of Arcobacter were consecutively digested with HpaII, CfoI, and HinfI. A reproducible and discriminatory identification scheme based on combined restriction patterns was developed by using reference strains and was applied to the identification of a variety of isolates.


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