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Journal of Clinical Microbiology, January 1998, p. 128-132, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Enterobacterial Repetitive Intergenic Consensus
Sequences as Molecular Targets for Typing of Mycobacterium
tuberculosis Strains
Leonardo A.
Sechi,1,*
Stefania
Zanetti,1
Ilaria
Dupré,1
Giovanni
Delogu,1 and
Giovanni
Fadda2
Dipartimento di Scienze Biomediche, Sezione
di Microbiologia Sperimentale e Clinica, Università degli studi
di Sassari, 07100 Sassari,1 and
Istituto
di Microbiologia, Facoltà di Medicina e Chirurgia "Agostino
Gemelli," Università Cattolica del Sacro Cuore, 00168 Rome,2 Italy
Received 27 February 1997/Returned for modification 25 June
1997/Accepted 10 October 1997
The presence of enterobacterial repetitive intergenic consensus
(ERIC) sequences was demonstrated for the first time in the genome of
Mycobacterium tuberculosis; these sequences have been found
in transcribed regions of the chromosomes of gram-negative bacteria. In
this study genetic diversity among clinical isolates of M. tuberculosis was determined by PCR with ERIC primers (ERIC-PCR). The study isolates comprised 71 clinical isolates collected from Sardinia, Italy. ERIC-PCR was able to identify 59 distinct profiles. The results obtained were compared with IS6110 and PCR-GTG
fingerprinting. We found that the level of differentiation obtained by
ERIC-PCR is greater than that obtained by IS6110
fingerprinting and comparable to that obtained by PCR-GTG. This method
of fingerprinting is rapid and sensitive and can be applied to the
study of the epidemiology of M. tuberculosis infections,
especially when IS6110 fingerprinting is not of any help.
*
Corresponding author. Mailing address: Dipartimento di
Scienze Biomediche, Sezione di Microbiologia Sperimentale e Clinica, Università degli studi di Sassari, Viale S. Pietro 43/B, 07100 Sassari, Italy. Phone: 79 228303. Fax: 79 212345. E-mail:
microb{at}ssmain.uniss.it.
Journal of Clinical Microbiology, January 1998, p. 128-132, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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