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Journal of Clinical Microbiology, January 1998, p. 161-167, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Comparative Analysis of Infrequent-Restriction-Site PCR and Pulsed-Field Gel Electrophoresis for Epidemiological Typing of Legionella pneumophila Serogroup 1 Strains

Serge Riffard,* François Lo Presti, François Vandenesch, Françoise Forey, Monique Reyrolle, and Jerome Etienne

Centre National de Référence des Legionella, Laboratoire de Bactériologie, UPRES EA1655, Faculté de Médecine R.T.H. Laënnec, 69372 Lyon Cedex 08, France

Received 22 July 1997/Returned for modification 24 September 1997/Accepted 8 October 1997

Two methods were compared for the analysis of 48 unrelated and epidemiologically related Legionella pneumophila serogroup 1 isolates. These are the infrequent-restriction-site PCR (IRS-PCR) assay with adapters designed for XbaI and PstI restriction sites and the pulsed-field gel electrophoresis (PFGE) analysis determined after DNA restriction with SfiI. Both methods demonstrated a high level of discrimination with a similar capacity for differentiating 23 of the 24 unrelated isolates. PFGE analysis and IRS-PCR assay were both able to identify epidemiologically related isolates of L. pneumophila from three outbreaks. Hence, IRS-PCR assay appears to be a reproducible (intergel reproducibility, 100%) and discriminative (discriminatory index, >= 0.996) tool for typing of Legionella. Compared to PFGE, however, IRS-PCR presented an advantage through ease of performance and with attributes of rapidity and sensitivity of target DNA.

* Corresponding author. Mailing address: UPRES EA1655, Faculté de Médecine R.T.H. Laënnec, rue Guillaume Paradin, 69372 Lyon Cedex 08, France. Phone: (33) 478 77 86 57. Fax: (33) 478 77 86 58. E-mail: derba{at}cimac-res.univ-lyon1.fr.

Journal of Clinical Microbiology, January 1998, p. 161-167, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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