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Journal of Clinical Microbiology, January 1998, p. 161-167, Vol. 36, No. 1
Centre National de Référence des
Legionella, Laboratoire de Bactériologie, UPRES EA1655,
Faculté de Médecine R.T.H. Laënnec, 69372 Lyon Cedex
08, France
Received 22 July 1997/Returned for modification 24 September
1997/Accepted 8 October 1997
Two methods were compared for the analysis of 48 unrelated and
epidemiologically related Legionella pneumophila serogroup 1 isolates. These are the infrequent-restriction-site PCR (IRS-PCR) assay with adapters designed for XbaI and PstI
restriction sites and the pulsed-field gel electrophoresis (PFGE)
analysis determined after DNA restriction with SfiI. Both
methods demonstrated a high level of discrimination with a similar
capacity for differentiating 23 of the 24 unrelated isolates. PFGE
analysis and IRS-PCR assay were both able to identify epidemiologically
related isolates of L. pneumophila from three outbreaks.
Hence, IRS-PCR assay appears to be a reproducible (intergel
reproducibility, 100%) and discriminative (discriminatory index,
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Comparative Analysis of Infrequent-Restriction-Site
PCR and Pulsed-Field Gel Electrophoresis for Epidemiological Typing of
Legionella pneumophila Serogroup 1 Strains
0.996) tool for typing of Legionella. Compared to PFGE,
however, IRS-PCR presented an advantage through ease of performance and
with attributes of rapidity and sensitivity of target DNA.
*
Corresponding author. Mailing address: UPRES EA1655,
Faculté de Médecine R.T.H. Laënnec, rue Guillaume
Paradin, 69372 Lyon Cedex 08, France. Phone: (33) 478 77 86 57. Fax:
(33) 478 77 86 58. E-mail:
derba{at}cimac-res.univ-lyon1.fr.
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