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Journal of Clinical Microbiology, January 1998, p. 251-254, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Development of an Immunomagnetic Method for Selective Isolation of Actinobacillus pleuropneumoniae Serotype 1 from Tonsils

Annie Gagné,1 Sonia Lacouture,1 André Broes,2 Sylvie D'Allaire,1 and Marcelo Gottschalk1,*

Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada J2S 7C6,1 and Centre de Développement du Porc du Québec, Sainte-Foy, Québec, Canada G1V 4M42

Received 19 May 1997/Returned for modification 29 July 1997/Accepted 2 October 1997

An immunomagnetic separation technique (IMS) for the selective isolation of Actinobacillus pleuropneumoniae serotype 1 was developed. Superparamagnetic polystyrene beads (immunomagnetic beads [IMBs]) were coated with purified rabbit immunoglobulin G specific for A. pleuropneumoniae serotype 1. The antibody concentration, the number of IMBs, the incubation time, and the temperature of incubation influenced the recovery of the target bacteria. The sensitivity of the IMS technique was 1,000-fold higher than that of direct culture. When tonsils from animals from infected herds were tested, significantly more positive tonsils were detected by the IMS technique (68%) than by the standard procedures (22%). The method represents an innovative and highly sensitive approach for the isolation of A. pleuropneumoniae from carrier animals.

* Corresponding author. Mailing address: Faculté de Médecine Vétérinaire, Université de Montréal, C.P. 5000, St-Hyacinthe, Québec, Canada J2S 7C6. Phone: (514) 773-8521, ext. 8374. Fax: (514) 778-8108. E-mail: gottschm{at}ere.umontreal.ca.

Journal of Clinical Microbiology, January 1998, p. 251-254, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.