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Journal of Clinical Microbiology, January 1998, p. 258-260, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma by Using Blood Dried on Filter Paper

Susan A. Fiscus,1,* Donald Brambilla,2 Lynell Grosso,1 Jody Schock,1 and Michael Cronin3

Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina1; New England Research Institute, Watertown, Massachusetts2; and Organon Teknika, Durham, North Carolina3

Received 30 May 1997/Returned for modification 30 July 1997/Accepted 1 October 1997

We compared whole blood dried on filter paper to the standard assay with frozen cell-free plasma for use in the quantitation of the human immunodeficiency virus RNA load in blood. RNA values from filter paper, corrected for the hematocrit, gave results comparable to those of the standard assay in terms of sensitivity and reproducibility.

* Corresponding author. Mailing address: University of North Carolina at Chapel Hill, CB# 7140, Chapel Hill, NC 27599-7140. Phone: (919) 966-6872. Fax: (919) 966-9873. E-mail: sfiscus{at}email.unc.edu.

Journal of Clinical Microbiology, January 1998, p. 258-260, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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