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Journal of Clinical Microbiology, January 1998, p. 275-276, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Inhibition of PCR by Agar from Bacteriological Transport Media

Alan Patrick Gibb1,2,3,* and Sallene Wong1

Provincial Laboratory for Public Health of Southern Alberta,1 Department of Microbiology and Infectious Diseases, University of Calgary,2 and Department of Microbiology and Infectious Diseases, Foothills Hospital,3 Calgary, Alberta, Canada

Received 23 June 1997/Returned for modification 2 September 1997/Accepted 10 October 1997

We observed inhibition of PCR in throat swabs submitted in routine bacteriological transport media. Experimental studies showed that agar, which was extracted with DNA by DNAzol (Gibco BRL, Gaithersburg, Md.), was the inhibitory agent. No inhibitory effect was observed with a transport medium which did not contain agar.

* Corresponding author. Mailing address: Department of Microbiology and Infectious Diseases, Foothills Hospital, 1403-29 Street, Calgary, Alberta, Canada T2N 2T9. Phone: (403) 670-2584. Fax: (403) 270-2216. E-mail: gibb{at}acs.ucalgary.ca.

Journal of Clinical Microbiology, January 1998, p. 275-276, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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