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Journal of Clinical Microbiology, January 1998, p. 321-323, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection of Helicobacter pylori in Fecal Samples of Gnotobiotic Mice Infected with H. pylori by an Immunomagnetic-Bead Separation Technique

Takako Osaki,1,* Haruhiko Taguchi,2 Hiroyuki Yamaguchi,2 and Shigeru Kamiya2

Division of Flow Cytometry1 and Department of Microbiology,2 Kyorin University School of Medicine, Mitaka, Japan

Received 9 June 1997/Returned for modification 4 August 1997/Accepted 27 October 1997

By an immunomagnetic-bead (IMB) separation technique, isolation of Helicobacter pylori from gastrointestinal and fecal samples of gnotobiotic mice infected with the microorganism was tried. The isolation rate of H. pylori from stomach samples after IMB separation was not higher than that of direct culture of the samples. After IMB separation of feces, H. pylori was detectable by PCR, although H. pylori was not culturable.

* Corresponding author. Mailing address: Department of Microbiology, Kyorin University School of Medicine, 6-20-2, Shinkawa, Mitaka 181, Japan. Phone: 81-422-47-5511, ext. 3464. Fax: 81-422-44-7325. E-mail: osaki{at}kyorin-u.ac.jp.

Journal of Clinical Microbiology, January 1998, p. 321-323, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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