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Journal of Clinical Microbiology, January 1998, p. 37-40, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification of Encephalitozoon intestinalis in Travelers with Chronic Diarrhea by Specific PCR Amplification

Laurent Raynaud,1,2 Frederic Delbac,3 Veronique Broussolle,3 Meja Rabodonirina,2 Veronique Girault,2 Martine Wallon,2 Gregoire Cozon,4 Christian P. Vivares,3 and François Peyron2,*

ESSA, 69500 Bron,1 Département de Parasitologie, Faculté de Médecine, Université Claude Bernard, 69373 Lyon Cedex 08,2 Protistologie Moléculaire et Cellulaire des Parasites Opportunistes, LBCP, ESA CNRS 6023, Université Blaise Pascal, 63177 Aubière Cedex,3 and Service Immunologie, Hôpital de la Croix Rousse, 69317 Lyon Cedex 04,4 France

Received 31 March 1997/Returned for modification 16 September 1997/Accepted 10 October 1997

With the use of Weber's modified trichrome and Uvitex 2B techniques, spores of microsporidia were detected in the stools of four travelers presenting clinically with chronic diarrhea. The general health of these patients was not impaired, and human immunodeficiency virus screening was negative. Immune evaluation, including the study of lymphocytic subpopulations, assay of serum immunoglobulins, and an intradermal multitest, showed normal results. Molecular identification of microsporidian species was based on the PCR amplification of a small-subunit rRNA sequence followed by HinfI endonuclease restriction. Encephalitozoon intestinalis microsporidiosis was thus shown in two of the four patients examined. In two patients, therapy based on albendazole made stools devoid of microsporidian spores without influence on the intestinal disorders. The pathogenic role of E. intestinalis in immunocompetent individuals remains to be demonstrated.

* Corresponding author. Mailing address: Département de Parasitologie, Faculté de Médecine, Université Claude Bernard, 8 Avenue Rockefeller 69373 Lyon Cedex 08, France. Phone: 33-04-78-77-70-00. Fax: 0033-04-78-75-17-72. E-mail: peyron{at}cismsun.univ-lyon1.fr.

Journal of Clinical Microbiology, January 1998, p. 37-40, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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