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Journal of Clinical Microbiology, January 1998, p. 68-72, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection of Human Herpesvirus 6 DNA in Serum by a Microplate PCR-Hybridization Assay

Carla Osiowy,* Isabelle Prud'homme,dagger Michael Monette, and Shimian Zou

Surveillance, Influenza and Viral Exanthemata, Bureau of Microbiology, Laboratory Centre for Disease Control, Tunney's Pasture, Ottawa, Ontario K1A 0L2, Canada

Received 12 May 1997/Returned for modification 27 August 1997/Accepted 20 October 1997

PCR was performed on DNA extracts derived from clinical serum samples submitted for human herpesvirus 6 (HHV-6) serological examination. To detect amplified HHV-6 products, a hybridization-based microtiter plate assay (PCR ELISA; Boehringer Mannheim) was used. The assay system was found to be rapid, specific, and sensitive. Approximately three copies of a plasmid-based HHV-6 sequence could be detected, and no cross amplification was observed with HHV-7 genomic DNA. There was no correlation found between HHV-6 DNA detection and serological status in clinical serum samples from individuals more than 2 years old. On the other hand, in serum samples from infants less than 2 years old, a high rate of detection of HHV-6 DNA was observed in those who lacked immunoglobulin G and M antibodies to HHV-6 (55%). In this regard, PCR of serum DNA extracts may be used as a sensitive indicator of active HHV-6 infection in infants prior to their seroconversion.

* Corresponding author. Mailing address: Health Canada, Bureau of Microbiology, Virus Laboratory, Building No. 10, Postal Locator 1001C, Tunney's Pasture, Ottawa, Ontario K1A 0L2, Canada. Phone: (613) 941-1465. Fax: (613) 954-0207. E-mail: carla_osiowy{at}inet.hwc.ca.

dagger Present address: HIV Reference Services Laboratory, Bureau of HIV/AIDS and STDs, Laboratory Centre for Disease Control, Tunney's Pasture, Ottawa, Ontario K1A 0L2, Canada.

Journal of Clinical Microbiology, January 1998, p. 68-72, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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