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Journal of Clinical Microbiology, October 1998, p. 2844-2846, Vol. 36, No. 10
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

PCR for Detection and Identification of Abiotrophia spp.

Andreas Roggenkamp,1,* Lorenz Leitritz,1 Kerstin Baus,1 Enevold Falsen,2 and Jürgen Heesemann1

Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University Munich, 80336 Munich, Germany,1 and Culture Collection, Department of Clinical Bacteriology, University of Göteborg, S-413 46 Göteborg, Sweden2

Received 21 April 1998/Returned for modification 23 June 1998/Accepted 21 July 1998

Members of the genus Abiotrophia, formerly known as nutritionally variant streptococci, are important pathogens causing septicemia and endocarditis. Cultivation and biochemical differentiation of Abiotrophia spp. are often difficult. Based on 16S rRNA sequences, two PCR assays for detection and identification of Abiotrophia spp. were developed. The first PCR assay was positive for all Abiotrophia spp. Subsequently performed restriction fragment length polymorphism analysis allowed the verification of the PCR amplicons and the differentiation of the three species. The second PCR assay was positive only for A. elegans, the most fastidious species of Abiotrophia. Both PCR assays were shown to be specific and sensitive and should facilitate the identification of Abiotrophia spp.

* Corresponding author. Mailing address: Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University Munich, Pettenkoferstr. 9a, 80336 Munich, Germany. Phone: 49-89-51605200. Fax: 49-89-51605202. E-mail: Rogge{at}m3401.mpk.med.uni-muenchen.de.

Journal of Clinical Microbiology, October 1998, p. 2844-2846, Vol. 36, No. 10
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.