Previous Article | Next Article 
Journal of Clinical Microbiology, October 1998, p. 2869-2873, Vol. 36, No. 10
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Prevalence of Candida dubliniensis Isolates in a
Yeast Stock Collection
Frank C.
Odds,*
Luc
Van Nuffel, and
Géry
Dams
Department of Bacteriology and Mycology,
Janssen Research Foundation, 2340 Beerse, Belgium
Received 2 April 1998/Returned for modification 26 June
1998/Accepted 10 July 1998
To establish the historical prevalence of the novel yeast
species Candida dubliniensis, a survey of 2,589 yeasts
originally identified as Candida albicans and maintained in
a stock collection dating back to the early 1970s was undertaken. A
total of 590 yeasts, including 93 (18.5%)
-glucosidase-negative
isolates among 502 isolates that showed abnormal colony colors on a
differential chromogenic agar and 497 other isolates, were subjected to
DNA fingerprinting with the moderately repetitive sequence Ca3. On this
basis, 53 yeasts were reidentified as C. dubliniensis
(including the C. dubliniensis type strain, included
as a blind control in the panel of yeasts). The 52 newly found isolates
came from 36 different persons, and a further 3 C. dubliniensis isolates were detected by DNA fingerprinting of
previously untested isolates from one of these individuals. The
prevalence of C. dubliniensis among yeasts in
oral and fecal samples was significantly higher than that among yeasts
from other anatomical sites and was significantly higher among human
immunodeficiency virus (HIV)-infected individuals than among known or
presumed HIV-negative individuals. However, a single vaginal isolate
and two oral isolates from healthy volunteers confirmed that the
species is restricted neither to gastrointestinal sites nor to patients
with overt disease. The oldest examples of C. dubliniensis were from oral samples of three patients in the
United Kingdom in 1973 and 1975. In comparison with age-matched control
isolates of C. albicans, the
C. dubliniensis isolates showed slightly higher levels
of susceptibility in vitro to amphotericin B and flucytosine and
slightly lower levels of susceptibility to three azole antifungal
agents.
*
Corresponding author. Mailing address: Department of
Bacteriology and Mycology, Janssen Research Foundation, B-2340 Beerse, Belgium. Phone: (32) 14-603004. Fax: (32) 14-605403. E-mail:
fodds{at}janbe.jnj.com.
Journal of Clinical Microbiology, October 1998, p. 2869-2873, Vol. 36, No. 10
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
-
Odds, F. C., Davidson, A. D., Jacobsen, M. D., Tavanti, A., Whyte, J. A., Kibbler, C. C., Ellis, D. H., Maiden, M. C. J., Shaw, D. J., Gow, N. A. R.
(2006). Candida albicans Strain Maintenance, Replacement, and Microvariation Demonstrated by Multilocus Sequence Typing.. J. Clin. Microbiol.
44: 3647-3658
[Abstract]
[Full Text]
-
Tavanti, A., Davidson, A. D., Fordyce, M. J., Gow, N. A. R., Maiden, M. C. J., Odds, F. C.
(2005). Population Structure and Properties of Candida albicans, as Determined by Multilocus Sequence Typing. J. Clin. Microbiol.
43: 5601-5613
[Abstract]
[Full Text]
-
Sahand, I. H., Moragues, M. D., Eraso, E., Villar-Vidal, M., Quindos, G., Ponton, J.
(2005). Supplementation of CHROMagar Candida Medium with Pal's Medium for Rapid Identification of Candida dubliniensis. J. Clin. Microbiol.
43: 5768-5770
[Abstract]
[Full Text]
-
Marot-Leblond, A., Grimaud, L., David, S., Sullivan, D. J., Coleman, D. C., Ponton, J., Robert, R.
(2004). Evaluation of a Rapid Immunochromatographic Assay for Identification of Candida albicans and Candida dubliniensis. J. Clin. Microbiol.
42: 4956-4960
[Abstract]
[Full Text]
-
Khan, Z. U., Ahmad, S., Mokaddas, E., Chandy, R.
(2004). Tobacco Agar, a New Medium for Differentiating Candida dubliniensis from Candida albicans. J. Clin. Microbiol.
42: 4796-4798
[Abstract]
[Full Text]
-
Ahmad, S., Khan, Z., Mokaddas, E., Khan, Z. U.
(2004). Isolation and molecular identification of Candida dubliniensis from non-human immunodeficiency virus-infected patients in Kuwait. J Med Microbiol
53: 633-637
[Abstract]
[Full Text]
-
Pinjon, E., Moran, G. P., Jackson, C. J., Kelly, S. L., Sanglard, D., Coleman, D. C., Sullivan, D. J.
(2003). Molecular Mechanisms of Itraconazole Resistance in Candida dubliniensis. Antimicrob. Agents Chemother.
47: 2424-2437
[Abstract]
[Full Text]
-
Kim, J. O., Garofalo, L., Blecker-Shelly, D., McGowan, K. L.
(2003). Candida dubliniensis Infections in a Pediatric Population: Retrospective Identification from Clinical Laboratory Isolates of Candida albicans. J. Clin. Microbiol.
41: 3354-3357
[Abstract]
[Full Text]
-
Montour, L., Tey, R., Xu, J.
(2003). Isolation of Candida dubliniensis in an Aboriginal Community in Ontario, Canada. J. Clin. Microbiol.
41: 3423-3426
[Abstract]
[Full Text]
-
Blignaut, E., Pujol, C., Joly, S., Soll, D. R.
(2003). Racial Distribution of Candida dubliniensis Colonization among South Africans. J. Clin. Microbiol.
41: 1838-1842
[Abstract]
[Full Text]
-
Sancak, B., Rex, J. H., Paetznick, V., Chen, E., Rodriguez, J.
(2003). Evaluation of a Method for Identification of Candida dubliniensis Bloodstream Isolates. J. Clin. Microbiol.
41: 489-491
[Abstract]
[Full Text]
-
Perea, S., Lopez-Ribot, J. L., Wickes, B. L., Kirkpatrick, W. R., Dib, O. P., Bachmann, S. P., Keller, S. M., Martinez, M., Patterson, T. F.
(2002). Molecular Mechanisms of Fluconazole Resistance in Candida dubliniensis Isolates from Human Immunodeficiency Virus-Infected Patients with Oropharyngeal Candidiasis. Antimicrob. Agents Chemother.
46: 1695-1703
[Abstract]
[Full Text]
-
Ramage, G., Vande Walle, K., Wickes, B. L., Lopez-Ribot, J. L.
(2001). Biofilm Formation by Candida dubliniensis. J. Clin. Microbiol.
39: 3234-3240
[Abstract]
[Full Text]
-
Guiver, M, Levi, K, Oppenheim, B A
(2001). Rapid identification of candida species by TaqMan PCR. J. Clin. Pathol.
54: 362-366
[Abstract]
[Full Text]
-
Stevens, D. A., Coleman, D. C., Sullivan, D. J.
(2001). First Report of Candida dubliniensis in the Middle East. J. Clin. Microbiol.
39: 416-416
[Full Text]
-
Peltroche-Llacsahuanga, H., Schmidt, S., Seibold, M., Lütticken, R., Haase, G.
(2000). Differentiation between Candida dubliniensis and Candida albicans by Fatty Acid Methyl Ester Analysis Using Gas-Liquid Chromatography. J. Clin. Microbiol.
38: 3696-3704
[Abstract]
[Full Text]
-
Meis, J. F. G. M., Verduyn Lunel, F. M., Verweij, P. E., Voss;, A., Coleman, D.
(2000). One-Year Prevalence of Candida dublinienis in a Dutch University Hospital. J. Clin. Microbiol.
38: 3139-3140
[Full Text]
-
Chen, Y. C., Eisner, J. D., Kattar, M. M., Rassoulian-Barrett, S. L., LaFe, K., Yarfitz, S. L., Limaye, A. P., Cookson, B. T.
(2000). Identification of Medically Important Yeasts Using PCR-Based Detection of DNA Sequence Polymorphisms in the Internal Transcribed Spacer 2 Region of the rRNA Genes. J. Clin. Microbiol.
38: 2302-2310
[Abstract]
[Full Text]
-
Jabra-Rizk, M. A., Falkler, W. A. Jr., Merz, W. G., Baqui, A. A. M. A., Kelley, J. I., Meiller, T. F.
(2000). Retrospective Identification and Characterization of Candida dubliniensis Isolates among Candida albicans Clinical Laboratory Isolates from Human Immunodeficiency Virus (HIV)-Infected and Non-HIV-Infected Individuals. J. Clin. Microbiol.
38: 2423-2426
[Abstract]
[Full Text]
-
Tintelnot, K., Haase, G., Seibold, M., Bergmann, F., Staemmler, M., Franz, T., Naumann, D.
(2000). Evaluation of Phenotypic Markers for Selection and Identification of Candida dubliniensis. J. Clin. Microbiol.
38: 1599-1608
[Abstract]
[Full Text]
-
Polacheck, I., Strahilevitz, J., Sullivan, D., Donnelly, S., Salkin, I. F., Coleman, D. C.
(2000). Recovery of Candida dubliniensis from Non-Human Immunodeficiency Virus-Infected Patients in Israel. J. Clin. Microbiol.
38: 170-174
[Abstract]
[Full Text]
-
Gales, A. C., Pfaller, M. A., Houston, A. K., Joly, S., Sullivan, D. J., Coleman, D. C., Soll, D. R.
(1999). Identification of Candida dubliniensis Based on Temperature and Utilization of Xylose and alpha -Methyl-D-Glucoside as Determined with the API 20C AUX and Vitek YBC Systems. J. Clin. Microbiol.
37: 3804-3808
[Abstract]
[Full Text]
-
Pincus, D. H., Coleman, D. C., Pruitt, W. R., Padhye, A. A., Salkin, I. F., Geimer, M., Bassel, A., Sullivan, D. J., Clarke, M., Hearn, V.
(1999). Rapid Identification of Candida dubliniensis with Commercial Yeast Identification Systems. J. Clin. Microbiol.
37: 3533-3539
[Abstract]
[Full Text]
-
Jabra-Rizk, M. A., Falkler, W. A. Jr., Merz, W. G., Kelley, J. I., Baqui, A. A. M. A., Meiller, T. F.
(1999). Coaggregation of Candida dubliniensis with Fusobacterium nucleatum. J. Clin. Microbiol.
37: 1464-1468
[Abstract]
[Full Text]
-
Jabra-Rizk, M. A., Baqui, A. A. M. A., Kelley, J. I., Falkler, W. A. Jr., Merz, W. G., Meiller, T. F.
(1999). Identification of Candida dubliniensis in a Prospective Study of Patients in the United States. J. Clin. Microbiol.
37: 321-326
[Abstract]
[Full Text]
-
Koehler, A. P., Chu, K.-C., Houang, E. T. S., Cheng, A. F. B.
(1999). Simple, Reliable, and Cost-Effective Yeast Identification Scheme for the Clinical Laboratory. J. Clin. Microbiol.
37: 422-426
[Abstract]
[Full Text]