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Journal of Clinical Microbiology, October 1998, p. 2970-2972, Vol. 36, No. 10
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Field Validation of Laboratory Tests for Clinical Diagnosis of Sheep-Associated Malignant Catarrhal Fever

U. U. Müller-Doblies,1 H. Li,2 B. Hauser,3 H. Adler,1 and M. Ackermann1,*

Institute of Virology1 and Institute of Veterinary Pathology,3 University of Zürich, Zürich, Switzerland, and Animal Disease Research Unit, USDA-ARS, and Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington2

Received 11 February 1998/Returned for modification 4 June 1998/Accepted 23 June 1998

Until recently, sheep-associated malignant catarrhal fever (SA-MCF) was diagnosed mainly on the basis of clinical presentation and histopathological changes. Using clinically diagnosed field cases, we have evaluated a seminested PCR and a competitive inhibition enzyme-linked immunosorbent assay (CI-ELISA) and compared these assays in the diagnosis of SA-MCF in cattle with histopathology as a provisional "gold standard." Samples from 44 cattle with clinical signs suggestive of SA-MCF were examined by histopathology, PCR, and CI-ELISA. In addition, samples from healthy cattle were evaluated by PCR (n = 96) and CI-ELISA (n = 75). Based on histopathology, 38 of the 44 clinical cases were classified as SA-MCF positive, 3 were classified as inconclusive, and 3 were classified as SA-MCF negative. The sensitivity of PCR was 95 to 97%, whereas the specificity ranged between 94 and 100%. The CI-ELISA showed a sensitivity of 56 to 87% and a specificity between 91 and 100%. In the field, there is good correlation between the diagnoses of SA-MCF by histopathology, PCR, and CI-ELISA. These data also confirm the close association of ovine herpesvirus 2 with SA-MCF in Switzerland.

* Corresponding author. Mailing address: Virologisches Institut, Universität Zürich, Winterthurerstr. 266a, 8057 Zürich, Switzerland. Phone: 41-1-635 87 01. Fax: 41-1-635 89 11. E-mail: ma{at}vetvir.unizh.ch.

Journal of Clinical Microbiology, October 1998, p. 2970-2972, Vol. 36, No. 10
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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