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Journal of Clinical Microbiology, October 1998, p. 3048-3050, Vol. 36, No. 10
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Evaluation of a Selective Transport Medium for Gastric Biopsy Specimens To Be Cultured for Helicobacter pylori

L. K. Siu,1 W. K. Leung,2 A. F. B. Cheng,1 J. Y. Sung,2 T. K. W. Ling,1,* J. M. L. Ling,1 E. K. W. Ng,3 J. Y. W. Lau,3 and S. C. S. Chung3

Departments of Microbiology,1 Medicine and Therapeutics,2 and Surgery,3 Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, China

Received 18 March 1998/Returned for modification 5 May 1998/Accepted 7 July 1998

Since the means of culturing Helicobacter pylori may not be available in some laboratories, prolonging the survival of this organism during transportation is a major concern in terms of improving detection rates. A selective transport medium was evaluated for the preservation of H. pylori from 254 gastric biopsy specimens collected from a rural area in China where culturing is not feasible. Gastric biopsy specimens were inoculated in sterile broth consisting of brain heart infusion (BHI) broth, horse serum, and yeast extract supplemented with vancomycin, amphotericin B, and nalidixic acid (VAN). Of the 254 biopsy specimens, 238 were identified by histology to have H. pylori infection. Total rates of recovery of H. pylori from the H. pylori-positive gastric biopsy specimens stored in the BHI-VAN broth ranged from 76 to 46% after storage of specimens for 5 to 9 days. In conclusion, the selective medium is useful for prolonging the survival of H. pylori in gastric biopsy specimens for which immediate culture is not feasible.

* Corresponding author. Mailing address: Department of Microbiology, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong (SAR), China. Phone: (852) 2632-3333. Fax: (852) 2647-3227. E-mail: lingt{at}sco1.med.cuhk.edu.hk.

Journal of Clinical Microbiology, October 1998, p. 3048-3050, Vol. 36, No. 10
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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