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Journal of Clinical Microbiology, October 1998, p. 3077-3078, Vol. 36, No. 10
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Genotyping of Chlamydia trachomatis in Urine Specimens Will Facilitate Large Epidemiological Studies

Servaas A. Morré,1 Robert Moes,1 Irene Van Valkengoed,2 Joan P. Boeke,2 Jacques T. M. van Eijk,2 Chris J. L. M. Meijer,1 and Adriaan J. C. Van den Brule1,*

Section of Molecular Pathology, Department of Pathology, University Hospital Vrije Universiteit, 1081 HV,1 and Institute for Research in Extramural Medicine, Vrije Universiteit, 1081 BT,2 Amsterdam, The Netherlands

Received 3 April 1998/Returned for modification 9 June 1998/Accepted 17 July 1998

The serovars of Chlamydia trachomatis-positive urine specimens (n = 81; as detected by PCR and ligase chain reaction) were successfully analyzed in 94% of cases by omp1 PCR-based RFLP analysis. The use of urine specimens and this simple and sensitive typing method will greatly facilitate epidemiological studies of C. trachomatis serovar distribution in asymptomatic C. trachomatis infections in both females and males.

* Corresponding author. Mailing address: Section of Molecular Pathology, Department of Pathology, University Hospital Vrije Universiteit, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands. Phone: 31-20-4440503 or 31-20-44023. Fax: 31-20-4442964. E-mail: vandenbrule{at}azvu.nl.

Journal of Clinical Microbiology, October 1998, p. 3077-3078, Vol. 36, No. 10
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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