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Journal of Clinical Microbiology, October 1998, p. 3079-3080, Vol. 36, No. 10
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection of Aspergillus Galactomannan: Comparison of an Enzyme-Linked Immunoassay and a Europium-Linked Time-Resolved Fluoroimmunoassay

A. Paugam,1,* J. Sarfati,2 R. Romieu,3 M. Viguier,3 J. Dupouy-Camet,1 and J. P. Latgé2

Laboratoire de Mycologie1 and INSERM 445,3 Hôpital Cochin, and Laboratoire des Aspergillus, Institut Pasteur,2 Paris, France

Received 4 May 1998/Returned for modification 8 June 1998/Accepted 7 July 1998

With a view to improving the sensitivity of serological detection of Aspergillus galactomannan (GM), a europium-linked time-resolved fluoroimmunoassay was developed. This method was compared to an enzyme-linked immunosorbent assay using a peroxidase-conjugated detector antibody. No increase in the sensitivity of the detection of GM standards was seen with the europium-based fluoroimmunoassay.

* Corresponding author. Mailing address: Laboratoire de Mycologie, Hôpital Cochin, 75014 Paris, France. Phone: 1 42 34 14 97. Fax: 1 42 34 14 96. E-mail: andre.paugam{at}cch.ap-hop-paris.fr.

Journal of Clinical Microbiology, October 1998, p. 3079-3080, Vol. 36, No. 10
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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